3D/4D multiscale imaging in acute lymphoblastic leukemia cells: Visualizing dynamics of cell death

Sreelatha Sarangapani; Rosmin Elsa Mohan; Ajeetkumar Patil; Matthew J. Lang; Anand Asundi

doi:10.1117/12.2270979

3D/4D multiscale imaging in acute lymphoblastic leukemia cells: Visualizing dynamics of cell death

Sreelatha Sarangapani^*
, Rosmin Elsa Mohan
, Ajeetkumar Patil
, Matthew J. Lang
, Anand Asundi

^*Corresponding author for this work

Manipal Institute of Applied Physics (MIAP)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

Quantitative phase detection is a new methodology that provides quantitative information on cellular morphology to monitor the cell status, drug response and toxicity. In this paper the morphological changes in acute leukemia cells treated with chitosan were detected using d'Bioimager a robust imaging system. Quantitative phase image of the cells was obtained with numerical analysis. Results show that the average area and optical volume of the chitosan treated cells is significantly reduced when compared with the control cells, which reveals the effect of chitosan on the cancer cells. From the results it can be attributed that d'Bioimager can be used as a non-invasive imaging alternative to measure the morphological changes of the living cells in real time.

Original language	English
Title of host publication	Fifth International Conference on Optical and Photonics Engineering
Editors	Anand Krishna Asundi
Publisher	SPIE
Volume	10449
ISBN (Electronic)	9781510613720
DOIs	https://doi.org/10.1117/12.2270979
Publication status	Published - 01-01-2017
Event	5th International Conference on Optical and Photonics Engineering - Singapore, Singapore Duration: 04-04-2017 → 07-04-2017

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	10449
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	5th International Conference on Optical and Photonics Engineering
Country/Territory	Singapore
City	Singapore
Period	04-04-17 → 07-04-17

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.2270979

Cite this

Sarangapani, S., Mohan, R. E., Patil, A., Lang, M. J., & Asundi, A. (2017). 3D/4D multiscale imaging in acute lymphoblastic leukemia cells: Visualizing dynamics of cell death. In A. K. Asundi (Ed.), Fifth International Conference on Optical and Photonics Engineering (Vol. 10449). Article 1044936 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10449). SPIE. https://doi.org/10.1117/12.2270979

Sarangapani, Sreelatha ; Mohan, Rosmin Elsa ; Patil, Ajeetkumar et al. / 3D/4D multiscale imaging in acute lymphoblastic leukemia cells : Visualizing dynamics of cell death. Fifth International Conference on Optical and Photonics Engineering. editor / Anand Krishna Asundi. Vol. 10449 SPIE, 2017. (Proceedings of SPIE - The International Society for Optical Engineering).

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title = "3D/4D multiscale imaging in acute lymphoblastic leukemia cells: Visualizing dynamics of cell death",

abstract = "Quantitative phase detection is a new methodology that provides quantitative information on cellular morphology to monitor the cell status, drug response and toxicity. In this paper the morphological changes in acute leukemia cells treated with chitosan were detected using d'Bioimager a robust imaging system. Quantitative phase image of the cells was obtained with numerical analysis. Results show that the average area and optical volume of the chitosan treated cells is significantly reduced when compared with the control cells, which reveals the effect of chitosan on the cancer cells. From the results it can be attributed that d'Bioimager can be used as a non-invasive imaging alternative to measure the morphological changes of the living cells in real time.",

author = "Sreelatha Sarangapani and Mohan, \{Rosmin Elsa\} and Ajeetkumar Patil and Lang, \{Matthew J.\} and Anand Asundi",

note = "Publisher Copyright: {\textcopyright} 2017 SPIE.; 5th International Conference on Optical and Photonics Engineering ; Conference date: 04-04-2017 Through 07-04-2017",

year = "2017",

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doi = "10.1117/12.2270979",

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Sarangapani, S, Mohan, RE, Patil, A, Lang, MJ & Asundi, A 2017, 3D/4D multiscale imaging in acute lymphoblastic leukemia cells: Visualizing dynamics of cell death. in AK Asundi (ed.), Fifth International Conference on Optical and Photonics Engineering. vol. 10449, 1044936, Proceedings of SPIE - The International Society for Optical Engineering, vol. 10449, SPIE, 5th International Conference on Optical and Photonics Engineering, Singapore, Singapore, 04-04-17. https://doi.org/10.1117/12.2270979

3D/4D multiscale imaging in acute lymphoblastic leukemia cells: Visualizing dynamics of cell death. / Sarangapani, Sreelatha; Mohan, Rosmin Elsa; Patil, Ajeetkumar et al.
Fifth International Conference on Optical and Photonics Engineering. ed. / Anand Krishna Asundi. Vol. 10449 SPIE, 2017. 1044936 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10449).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - 3D/4D multiscale imaging in acute lymphoblastic leukemia cells

T2 - 5th International Conference on Optical and Photonics Engineering

AU - Sarangapani, Sreelatha

AU - Mohan, Rosmin Elsa

AU - Patil, Ajeetkumar

AU - Lang, Matthew J.

AU - Asundi, Anand

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Quantitative phase detection is a new methodology that provides quantitative information on cellular morphology to monitor the cell status, drug response and toxicity. In this paper the morphological changes in acute leukemia cells treated with chitosan were detected using d'Bioimager a robust imaging system. Quantitative phase image of the cells was obtained with numerical analysis. Results show that the average area and optical volume of the chitosan treated cells is significantly reduced when compared with the control cells, which reveals the effect of chitosan on the cancer cells. From the results it can be attributed that d'Bioimager can be used as a non-invasive imaging alternative to measure the morphological changes of the living cells in real time.

AB - Quantitative phase detection is a new methodology that provides quantitative information on cellular morphology to monitor the cell status, drug response and toxicity. In this paper the morphological changes in acute leukemia cells treated with chitosan were detected using d'Bioimager a robust imaging system. Quantitative phase image of the cells was obtained with numerical analysis. Results show that the average area and optical volume of the chitosan treated cells is significantly reduced when compared with the control cells, which reveals the effect of chitosan on the cancer cells. From the results it can be attributed that d'Bioimager can be used as a non-invasive imaging alternative to measure the morphological changes of the living cells in real time.

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M3 - Conference contribution

AN - SCOPUS:85034088413

VL - 10449

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Fifth International Conference on Optical and Photonics Engineering

A2 - Asundi, Anand Krishna

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Sarangapani S, Mohan RE, Patil A, Lang MJ, Asundi A. 3D/4D multiscale imaging in acute lymphoblastic leukemia cells: Visualizing dynamics of cell death. In Asundi AK, editor, Fifth International Conference on Optical and Photonics Engineering. Vol. 10449. SPIE. 2017. 1044936. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.2270979

3D/4D multiscale imaging in acute lymphoblastic leukemia cells: Visualizing dynamics of cell death

Abstract

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UN SDGs

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