TY - JOUR
T1 - A comprehensive review on LED-induced fluorescence in diagnostic pathology
AU - Mukunda, Darshan Chikkanayakanahalli
AU - Rodrigues, Jackson
AU - Joshi, Vijay Kumar
AU - Raghushaker, Chandavalli Ramappa
AU - Mahato, Krishna Kishore
N1 - Funding Information:
The authors would like to thank SERB , Department of Science and Technology (DST), Govt. of India, New Delhi (Sanction ID: EMR-2016-007700 ) and Department of Biotechnology (DBT) , Government of India, New Delhi (Sanction ID: BTPR/14776/Med/32/460/2015 ) for financial support. The authors would like to thank Manipal Academy of Higher Education (MAHE), Manipal, India, TIFAC-CORE in Pharmacogenomics, DST, Govt. of India, FIST, DST, Govt. of India and BiSEP, Govt. of Karnataka, and Manipal School of Life Sciences for infrastructure facilities. The authors extend their gratitude to thank Prof. K. Satyamoorthy, Director, Manipal School of Life Sciences, for his encouragement and support. The authors would also like to thank Dr. Nirmal Mazumder, Assistant professor, Mr. Subhash Chandra, Assistant Engineer, Department of Biophysics, MSLS, MAHE, Manipal for their support. The author, DCM, would like to thank the Indian Council of Medical Research (ICMR), ITR division, Government of India, New Delhi , for granting ICMR-SRF (Sanction no. 5/3/8/14/ITR-F/2020-ITR ) to him. The authors, JR and CRR, would also like to thank ICMR, Government of India, New Delhi , for granting ICMR-SRFs (Sanction no. 5/3/8/45/ITR-F/2019-ITR and 5/3/8/60/ITR-F/2018-ITR ) to them. The authors would like to thank Ms. Sushmitha Srinivasan and Ms. Meagan Gail D'Souza for their constructive criticism.
Funding Information:
The authors would like to thank SERB, Department of Science and Technology (DST), Govt. of India, New Delhi (Sanction ID: EMR-2016-007700) and Department of Biotechnology (DBT), Government of India, New Delhi (Sanction ID: BTPR/14776/Med/32/460/2015) for financial support. The authors would like to thank Manipal Academy of Higher Education (MAHE), Manipal, India, TIFAC-CORE in Pharmacogenomics, DST, Govt. of India, FIST, DST, Govt. of India and BiSEP, Govt. of Karnataka, and Manipal School of Life Sciences for infrastructure facilities. The authors extend their gratitude to thank Prof. K. Satyamoorthy, Director, Manipal School of Life Sciences, for his encouragement and support. The authors would also like to thank Dr. Nirmal Mazumder, Assistant professor, Mr. Subhash Chandra, Assistant Engineer, Department of Biophysics, MSLS, MAHE, Manipal for their support. The author, DCM, would like to thank the Indian Council of Medical Research (ICMR), ITR division, Government of India, New Delhi, for granting ICMR-SRF (Sanction no.5/3/8/14/ITR-F/2020-ITR) to him. The authors, JR and CRR, would also like to thank ICMR, Government of India, New Delhi, for granting ICMR-SRFs (Sanction no. 5/3/8/45/ITR-F/2019-ITR and 5/3/8/60/ITR-F/2018-ITR) to them. The authors would like to thank Ms. Sushmitha Srinivasan and Ms. Meagan Gail D'Souza for their constructive criticism.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/8/1
Y1 - 2022/8/1
N2 - Sensitivity, specificity, mobility, and affordability are important criteria to consider for developing diagnostic instruments in common use. Fluorescence spectroscopy has been demonstrating substantial potential in the clinical diagnosis of diseases and evaluating the underlying causes of pathogenesis. A higher degree of device integration with appropriate sensitivity and reasonable cost would further boost the value of the fluorescence techniques in clinical diagnosis and aid in the reduction of healthcare expenses, which is a key economic concern in emerging markets. Light-emitting diodes (LEDs), which are inexpensive and smaller are attractive alternatives to conventional excitation sources in fluorescence spectroscopy, are gaining a lot of momentum in the development of affordable, compact analytical instruments of clinical relevance. The commercial availability of a broad range of LED wavelengths (255–4600 nm) has opened up new avenues for targeting a wide range of clinically significant molecules (both endogenous and exogenous), thereby diagnosing a range of clinical illnesses. As a result, we have specifically examined the uses of LED-induced fluorescence (LED-IF) in preclinical and clinical evaluations of pathological conditions, considering the present advancements in the field.
AB - Sensitivity, specificity, mobility, and affordability are important criteria to consider for developing diagnostic instruments in common use. Fluorescence spectroscopy has been demonstrating substantial potential in the clinical diagnosis of diseases and evaluating the underlying causes of pathogenesis. A higher degree of device integration with appropriate sensitivity and reasonable cost would further boost the value of the fluorescence techniques in clinical diagnosis and aid in the reduction of healthcare expenses, which is a key economic concern in emerging markets. Light-emitting diodes (LEDs), which are inexpensive and smaller are attractive alternatives to conventional excitation sources in fluorescence spectroscopy, are gaining a lot of momentum in the development of affordable, compact analytical instruments of clinical relevance. The commercial availability of a broad range of LED wavelengths (255–4600 nm) has opened up new avenues for targeting a wide range of clinically significant molecules (both endogenous and exogenous), thereby diagnosing a range of clinical illnesses. As a result, we have specifically examined the uses of LED-induced fluorescence (LED-IF) in preclinical and clinical evaluations of pathological conditions, considering the present advancements in the field.
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U2 - 10.1016/j.bios.2022.114230
DO - 10.1016/j.bios.2022.114230
M3 - Review article
AN - SCOPUS:85127732344
SN - 0956-5663
VL - 209
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 114230
ER -