TY - JOUR
T1 - A herbal combination attenuates muscle atrophy and cancer cachexia
T2 - A preclinical study
AU - Azeemuddin, Mohammed Mukhram
AU - Rao, Chamallamudi Mallikarjuna
AU - Rafiq, Mohamed
AU - Onkaramurthy, Mallappa
AU - Singh, Prachi
AU - Baig, Mirza Rizwan
AU - Babu, Uddagiri Venkanna
N1 - Funding Information:
The authors acknowledge the Phytochemistry, Natural Product Innovations, and Preclinical Pharmacology and Toxicology Departments, R&D Center, Himalaya Wellness Company, for providing support during the study.
Publisher Copyright:
© 2022. Mohammed Mukhram Azeemuddin et al. All Rights Reserved.
PY - 2022/4
Y1 - 2022/4
N2 - Cachexia is a life-threatening illness that affects up to 80% of cancer patients. The study aimed to evaluate the efficacy of herbal combination containing Boswellia serrata oleo-gum resin, Cissus quadrangularis stem and Withania somnifera root extracts (HIM-CHX) in experimental cachexia models. Muscle atrophy was assessed using C2C12 cells (skeletal muscle cells) and cancer cachexia was induced in female Wistar rats using DMBA (7, 12-dimethylbenz[a]anthracene). HIM-CHX was evaluated at three different doses: 125, 250, and 500 mg/kg of body weight. The length of the myotubes in C2C12 cells treated with cisplatin was significantly reduced which was increased by the addition of HIM-CHX. When DMBA-induced cachectic rats were given HIM-CHX, their body weight, feed consumption, muscle mass and longevity increased significantly and tumour necrosis factor TNI·'-α, interleukin (IL)-6, and myostatin decreased. The expression of muscle ring finger (MuRF)-1 gene was downregulated in the gastrocnemius muscle and the expression of insulin-like growth factor (IGI)-1 and IL-15 genes were upregulated. According to histological assessment, muscle atrophy improved in a dose-dependent manner. HIM-CHX regulates proinflammatory cytokines, myokines and genes involved in protein synthesis and proteolysis which helps to cure cachexia.
AB - Cachexia is a life-threatening illness that affects up to 80% of cancer patients. The study aimed to evaluate the efficacy of herbal combination containing Boswellia serrata oleo-gum resin, Cissus quadrangularis stem and Withania somnifera root extracts (HIM-CHX) in experimental cachexia models. Muscle atrophy was assessed using C2C12 cells (skeletal muscle cells) and cancer cachexia was induced in female Wistar rats using DMBA (7, 12-dimethylbenz[a]anthracene). HIM-CHX was evaluated at three different doses: 125, 250, and 500 mg/kg of body weight. The length of the myotubes in C2C12 cells treated with cisplatin was significantly reduced which was increased by the addition of HIM-CHX. When DMBA-induced cachectic rats were given HIM-CHX, their body weight, feed consumption, muscle mass and longevity increased significantly and tumour necrosis factor TNI·'-α, interleukin (IL)-6, and myostatin decreased. The expression of muscle ring finger (MuRF)-1 gene was downregulated in the gastrocnemius muscle and the expression of insulin-like growth factor (IGI)-1 and IL-15 genes were upregulated. According to histological assessment, muscle atrophy improved in a dose-dependent manner. HIM-CHX regulates proinflammatory cytokines, myokines and genes involved in protein synthesis and proteolysis which helps to cure cachexia.
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U2 - 10.7324/JAPS.2022.120413
DO - 10.7324/JAPS.2022.120413
M3 - Article
AN - SCOPUS:85129250249
SN - 2231-3354
VL - 12
SP - 119
EP - 126
JO - Journal of Applied Pharmaceutical Science
JF - Journal of Applied Pharmaceutical Science
IS - 4
ER -
