A herbal combination attenuates muscle atrophy and cancer cachexia: A preclinical study

Mohammed Mukhram Azeemuddin, Chamallamudi Mallikarjuna Rao, Mohamed Rafiq, Mallappa Onkaramurthy, Prachi Singh, Mirza Rizwan Baig, Uddagiri Venkanna Babu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Cachexia is a life-threatening illness that affects up to 80% of cancer patients. The study aimed to evaluate the efficacy of herbal combination containing Boswellia serrata oleo-gum resin, Cissus quadrangularis stem and Withania somnifera root extracts (HIM-CHX) in experimental cachexia models. Muscle atrophy was assessed using C2C12 cells (skeletal muscle cells) and cancer cachexia was induced in female Wistar rats using DMBA (7, 12-dimethylbenz[a]anthracene). HIM-CHX was evaluated at three different doses: 125, 250, and 500 mg/kg of body weight. The length of the myotubes in C2C12 cells treated with cisplatin was significantly reduced which was increased by the addition of HIM-CHX. When DMBA-induced cachectic rats were given HIM-CHX, their body weight, feed consumption, muscle mass and longevity increased significantly and tumour necrosis factor TNI·'-α, interleukin (IL)-6, and myostatin decreased. The expression of muscle ring finger (MuRF)-1 gene was downregulated in the gastrocnemius muscle and the expression of insulin-like growth factor (IGI)-1 and IL-15 genes were upregulated. According to histological assessment, muscle atrophy improved in a dose-dependent manner. HIM-CHX regulates proinflammatory cytokines, myokines and genes involved in protein synthesis and proteolysis which helps to cure cachexia.

Original languageEnglish
Pages (from-to)119-126
Number of pages8
JournalJournal of Applied Pharmaceutical Science
Issue number4
Publication statusPublished - 04-2022

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)


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