A method to score micronuclei in vivo using cytochalasin B-induced cytokinesis block

P. Uma Devi; B. S. Satish Rao; Ravindra Kamath

doi:10.1016/S0027-5107(97)00227-3

A method to score micronuclei in vivo using cytochalasin B-induced cytokinesis block

P. Uma Devi, B. S. Satish Rao, Ravindra Kamath

Department of Radiation Biology and Toxicology, Manipal School of Life Sciences, Manipal

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The present paper describes an in vivo micronucleus assay using Cytochalasin B (CyB). Mice bearing three different tumours, fibrosarcoma (Swiss albino mice), B16 F1 melanoma (C57 BL) and Ehrlich ascites carcinoma (Swiss albino mice), were injected with repeated doses of CyB at different time intervals and binucleate cells were scored at 24, 36, 48, 60 and 72 h after CyB injection. It was found that three doses of 3 + 2 + 2 mg/kg CyB administered intraperitoneally (i.p.) at 12-h intervals effectively blocked cytokinesis. The maximum number of binucleated cells (BNC) was scored at 60 h after the last CyB dose. This dose schedule was also effective in scoring micronuclei in BNC after irradiation.

Original language	English
Pages (from-to)	33-37
Number of pages	5
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	401
Issue number	1-2
DOIs	https://doi.org/10.1016/S0027-5107(97)00227-3
Publication status	Published - 05-06-1998

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

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10.1016/S0027-5107(97)00227-3

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title = "A method to score micronuclei in vivo using cytochalasin B-induced cytokinesis block",

abstract = "The present paper describes an in vivo micronucleus assay using Cytochalasin B (CyB). Mice bearing three different tumours, fibrosarcoma (Swiss albino mice), B16 F1 melanoma (C57 BL) and Ehrlich ascites carcinoma (Swiss albino mice), were injected with repeated doses of CyB at different time intervals and binucleate cells were scored at 24, 36, 48, 60 and 72 h after CyB injection. It was found that three doses of 3 + 2 + 2 mg/kg CyB administered intraperitoneally (i.p.) at 12-h intervals effectively blocked cytokinesis. The maximum number of binucleated cells (BNC) was scored at 60 h after the last CyB dose. This dose schedule was also effective in scoring micronuclei in BNC after irradiation.",

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T1 - A method to score micronuclei in vivo using cytochalasin B-induced cytokinesis block

AU - Uma Devi, P.

AU - Satish Rao, B. S.

AU - Kamath, Ravindra

PY - 1998/6/5

Y1 - 1998/6/5

N2 - The present paper describes an in vivo micronucleus assay using Cytochalasin B (CyB). Mice bearing three different tumours, fibrosarcoma (Swiss albino mice), B16 F1 melanoma (C57 BL) and Ehrlich ascites carcinoma (Swiss albino mice), were injected with repeated doses of CyB at different time intervals and binucleate cells were scored at 24, 36, 48, 60 and 72 h after CyB injection. It was found that three doses of 3 + 2 + 2 mg/kg CyB administered intraperitoneally (i.p.) at 12-h intervals effectively blocked cytokinesis. The maximum number of binucleated cells (BNC) was scored at 60 h after the last CyB dose. This dose schedule was also effective in scoring micronuclei in BNC after irradiation.

AB - The present paper describes an in vivo micronucleus assay using Cytochalasin B (CyB). Mice bearing three different tumours, fibrosarcoma (Swiss albino mice), B16 F1 melanoma (C57 BL) and Ehrlich ascites carcinoma (Swiss albino mice), were injected with repeated doses of CyB at different time intervals and binucleate cells were scored at 24, 36, 48, 60 and 72 h after CyB injection. It was found that three doses of 3 + 2 + 2 mg/kg CyB administered intraperitoneally (i.p.) at 12-h intervals effectively blocked cytokinesis. The maximum number of binucleated cells (BNC) was scored at 60 h after the last CyB dose. This dose schedule was also effective in scoring micronuclei in BNC after irradiation.

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A method to score micronuclei in vivo using cytochalasin B-induced cytokinesis block

Abstract

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