A micro-Raman spectroscopy study of inflammatory condition of human cervix: Probing of tissues and blood plasma samples

Ajaya Kumar Barik, Sanoop Pavithran M, Mithun N, Muralidhar V. Pai, Rekha Upadhya, Abhilash K. Pai, Jijo Lukose, Santhosh Chidangil

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The present study explores the application of the micro-Raman spectroscopy technique to discriminate normal and cervicitis condition from cervical malignancy by analyzing the Raman signatures of tissues and plasma samples of the same subjects. The Raman peaks from tissue samples at 1026 cm−1,1298 cm−1 and 1243 cm−1 are attributed to glycogen, fatty acids and collagen and are found to be reliable signatures capable of identifying cervicitis and normal condition from cervical cancer. The Raman signatures from plasma samples belonging to carbohydrates (578 cm−1), lipids (1059 cm−1) and nucleic acids (1077 cm−1,1341 cm−1 and 1357 cm−1) are quite useful to classify various pathological conditions of cervix at par with tissue based diagnosis. The PCA-SVM based classification of the spectral data indicates the potential of Raman spectroscopy based liquid biopsy to rule out false diagnosis of cervicitis as cervical malignancy.

Original languageEnglish
Article number102948
Pages (from-to)102948
JournalPhotodiagnosis and Photodynamic Therapy
Volume39
DOIs
Publication statusPublished - 09-2022

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Oncology
  • Dermatology
  • Pharmacology (medical)

Fingerprint

Dive into the research topics of 'A micro-Raman spectroscopy study of inflammatory condition of human cervix: Probing of tissues and blood plasma samples'. Together they form a unique fingerprint.

Cite this