A modified spectrophotometric micromethod to determine serum copper

Most of the available spectrophotometric methods to determine serum copper requires large volume of sample and involves several steps. We modified literature known method to estimate serum copper. Guanidine hydrocloride was used to release ceruloplasmin bound copper and determined using bathocuproine disulphonate disodium salt (BCDS). Our modified micromethod correlated well with the literature known method and shown good recovery and precision. Modified spectrophotometric micromethod is simple, sensitive, rapid, requires very less sample and coloring reagent and can be adopted in any clinical laboratory setups.