A modified spectrophotometric micromethod to determine serum copper

Mungli Prakash, Jeevan K. Shetty

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Most of the available spectrophotometric methods to determine serum copper requires large volume of sample and involves several steps. We modified literature known method to estimate serum copper. Guanidine hydrocloride was used to release ceruloplasmin bound copper and determined using bathocuproine disulphonate disodium salt (BCDS). Our modified micromethod correlated well with the literature known method and shown good recovery and precision. Modified spectrophotometric micromethod is simple, sensitive, rapid, requires very less sample and coloring reagent and can be adopted in any clinical laboratory setups.

Original languageEnglish
Pages (from-to)38-42
Number of pages5
JournalAsian Journal of Biochemistry
Issue number1
Publication statusPublished - 01-01-2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biochemistry, medical


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