A novel approach for phyto-synthesis of silver nanoparticles using floral extract of Punica granatum for potent anti-cancerous and antibacterial capabilities

This article explores the phyto-synthesis of silver nanoparticles (Ag NPs) derived from the floral extract of Punica granatum and their potential applications in antibacterial and anticancer treatments. In the synthesis process, silver nitrate (AgNO₃) has been utilized as both a reducing and stabilizing agent, leading to the successful formation of stable silver nanoparticles (PG-Ag NPs). The nanoparticles were characterized using various analytical techniques, revealing their spherical shape and uniform dispersion, with an average size of ~ 27 (± 2) nm. The antibacterial and anticancer properties of the PG-AgNPs have been investigated, demonstrating significant anticancer activity with an IC₅₀ value of ~ 13 µg/mL. Furthermore, the nanoparticles exhibited potent antimicrobial efficacy against Escherichia coli and Staphylococcus aureus. These findings indicate that PG-Ag NPs, synthesized from Punica granatum flower extract, present a promising and cost-effective solution for antibacterial and anticancer applications.