A Sensitive High Performance Liquid Chromatography Method for Determination of Bioequivalence of Atenolol Formulations

S. A. Shah; I. S. Rathod; C. J. Shishoo; S. S. Savale; M. C. Satia; K. M. Bhat

A Sensitive High Performance Liquid Chromatography Method for Determination of Bioequivalence of Atenolol Formulations

S. A. Shah, I. S. Rathod, C. J. Shishoo, S. S. Savale, M. C. Satia, K. M. Bhat

Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A sensitive and specific HPLC method with fluorescence detector has been developed for the analysis of atenolol in plasma using a reverse phase column (ODS, 250 × 4.6 mm, 10 μm). Atenolol levels were measured using its excitation and emission maxima at 276 nm and 302 nm, respectively. The method has a linearity range of 50-1000 ng/ml and recovery was found to be 95.7%. This method has been successfully used for the bioequivalence study of atenolol tablets (25 mg). C_max, t_max and AUC_0-∞ of the test formulation statistically does not show significant difference from the standard formulation.

Original language	English
Pages (from-to)	187-192
Number of pages	6
Journal	Indian Journal of Pharmaceutical Sciences
Volume	62
Issue number	3
Publication status	Published - 01-05-2000

All Science Journal Classification (ASJC) codes

Pharmaceutical Science

Cite this

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title = "A Sensitive High Performance Liquid Chromatography Method for Determination of Bioequivalence of Atenolol Formulations",

abstract = "A sensitive and specific HPLC method with fluorescence detector has been developed for the analysis of atenolol in plasma using a reverse phase column (ODS, 250 × 4.6 mm, 10 μm). Atenolol levels were measured using its excitation and emission maxima at 276 nm and 302 nm, respectively. The method has a linearity range of 50-1000 ng/ml and recovery was found to be 95.7%. This method has been successfully used for the bioequivalence study of atenolol tablets (25 mg). Cmax, tmax and AUC0-∞ of the test formulation statistically does not show significant difference from the standard formulation.",

author = "Shah, {S. A.} and Rathod, {I. S.} and Shishoo, {C. J.} and Savale, {S. S.} and Satia, {M. C.} and Bhat, {K. M.}",

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AU - Shah, S. A.

AU - Rathod, I. S.

AU - Shishoo, C. J.

AU - Savale, S. S.

AU - Satia, M. C.

AU - Bhat, K. M.

PY - 2000/5/1

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N2 - A sensitive and specific HPLC method with fluorescence detector has been developed for the analysis of atenolol in plasma using a reverse phase column (ODS, 250 × 4.6 mm, 10 μm). Atenolol levels were measured using its excitation and emission maxima at 276 nm and 302 nm, respectively. The method has a linearity range of 50-1000 ng/ml and recovery was found to be 95.7%. This method has been successfully used for the bioequivalence study of atenolol tablets (25 mg). Cmax, tmax and AUC0-∞ of the test formulation statistically does not show significant difference from the standard formulation.

AB - A sensitive and specific HPLC method with fluorescence detector has been developed for the analysis of atenolol in plasma using a reverse phase column (ODS, 250 × 4.6 mm, 10 μm). Atenolol levels were measured using its excitation and emission maxima at 276 nm and 302 nm, respectively. The method has a linearity range of 50-1000 ng/ml and recovery was found to be 95.7%. This method has been successfully used for the bioequivalence study of atenolol tablets (25 mg). Cmax, tmax and AUC0-∞ of the test formulation statistically does not show significant difference from the standard formulation.

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A Sensitive High Performance Liquid Chromatography Method for Determination of Bioequivalence of Atenolol Formulations

Abstract

All Science Journal Classification (ASJC) codes

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