A Sensitive High Performance Liquid Chromatography Method for Determination of Bioequivalence of Atenolol Formulations

S. A. Shah; I. S. Rathod; C. J. Shishoo; S. S. Savale; M. C. Satia; K. M. Bhat

A Sensitive High Performance Liquid Chromatography Method for Determination of Bioequivalence of Atenolol Formulations

S. A. Shah^*, I. S. Rathod, C. J. Shishoo, S. S. Savale, M. C. Satia, K. M. Bhat

^*Corresponding author for this work

Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A sensitive and specific HPLC method with fluorescence detector has been developed for the analysis of atenolol in plasma using a reverse phase column (ODS, 250 × 4.6 mm, 10 μm). Atenolol levels were measured using its excitation and emission maxima at 276 nm and 302 nm, respectively. The method has a linearity range of 50-1000 ng/ml and recovery was found to be 95.7%. This method has been successfully used for the bioequivalence study of atenolol tablets (25 mg). C_max, t_max and AUC_0-∞ of the test formulation statistically does not show significant difference from the standard formulation.

Original language	English
Pages (from-to)	187-192
Number of pages	6
Journal	Indian Journal of Pharmaceutical Sciences
Volume	62
Issue number	3
Publication status	Published - 01-05-2000

All Science Journal Classification (ASJC) codes

Pharmaceutical Science

Cite this

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title = "A Sensitive High Performance Liquid Chromatography Method for Determination of Bioequivalence of Atenolol Formulations",

abstract = "A sensitive and specific HPLC method with fluorescence detector has been developed for the analysis of atenolol in plasma using a reverse phase column (ODS, 250 × 4.6 mm, 10 μm). Atenolol levels were measured using its excitation and emission maxima at 276 nm and 302 nm, respectively. The method has a linearity range of 50-1000 ng/ml and recovery was found to be 95.7%. This method has been successfully used for the bioequivalence study of atenolol tablets (25 mg). Cmax, tmax and AUC0-∞ of the test formulation statistically does not show significant difference from the standard formulation.",

author = "Shah, {S. A.} and Rathod, {I. S.} and Shishoo, {C. J.} and Savale, {S. S.} and Satia, {M. C.} and Bhat, {K. M.}",

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T1 - A Sensitive High Performance Liquid Chromatography Method for Determination of Bioequivalence of Atenolol Formulations

AU - Shah, S. A.

AU - Rathod, I. S.

AU - Shishoo, C. J.

AU - Savale, S. S.

AU - Satia, M. C.

AU - Bhat, K. M.

PY - 2000/5/1

Y1 - 2000/5/1

N2 - A sensitive and specific HPLC method with fluorescence detector has been developed for the analysis of atenolol in plasma using a reverse phase column (ODS, 250 × 4.6 mm, 10 μm). Atenolol levels were measured using its excitation and emission maxima at 276 nm and 302 nm, respectively. The method has a linearity range of 50-1000 ng/ml and recovery was found to be 95.7%. This method has been successfully used for the bioequivalence study of atenolol tablets (25 mg). Cmax, tmax and AUC0-∞ of the test formulation statistically does not show significant difference from the standard formulation.

AB - A sensitive and specific HPLC method with fluorescence detector has been developed for the analysis of atenolol in plasma using a reverse phase column (ODS, 250 × 4.6 mm, 10 μm). Atenolol levels were measured using its excitation and emission maxima at 276 nm and 302 nm, respectively. The method has a linearity range of 50-1000 ng/ml and recovery was found to be 95.7%. This method has been successfully used for the bioequivalence study of atenolol tablets (25 mg). Cmax, tmax and AUC0-∞ of the test formulation statistically does not show significant difference from the standard formulation.

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SN - 0250-474X

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ER -

A Sensitive High Performance Liquid Chromatography Method for Determination of Bioequivalence of Atenolol Formulations

Abstract

All Science Journal Classification (ASJC) codes

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