A study on the protective effect of single dose of N-2- mercaptopropionyl glycine administration prior to renal reperfusion on wistar rats

N-2-mercaptopropionylglycine (NMG) is a powerful antioxidant which inhibits synthesize of superoxide radicals. It has been tested as a preventive agent against metabolic and structural damage induced during ischemia/reperfusion (I/R) process. To validate the efficacy of NMG on renal tissue this work was devised. During the experiment, the wistar strain rats were divided into five groups; the group I as normal control, the group II & III as I/R controls, and the group IV & V as I/R treated groups. The I/R control and I/R treated groups were subjected to ischemia and reperfusion either for 10 minutes (Gr.II & IV) or 90 min. (Gr.III & V). The I/R treated groups in addition received N-2- mercaptopropionylglycine (50mg/kg bw) intravenously prior to the onset of reperfusion. The results suggest that 60 minutes of ischemia followed by 10 (Gr.II) and 90 minutes (Gr.III) of reperfusion increased lipid peroxidation (MDA), and decreased glutathione (GSH), & Superoxide dismutase levels in the renal tissues compared to normal control. However, the GSH and SOD levels were higher and the lipid peroxidation level was lower in I/R treated groups than I/R controls. Thus, the present study confirms the protective effect of N-2- mercaptopropionylglycine on the reperfusion injury and also extends that protective effect on renal tissue.