TY - GEN
T1 - A sustainable bioprocess for lipase production using seawater and the byproduct obtained from coconut oil industries
AU - Raval, R.
AU - Verma, A.
AU - Raval, K.
N1 - Publisher Copyright:
© 2020 Taylor & Francis Group, London, ISBN 978-0-367-33737-7
PY - 2019
Y1 - 2019
N2 - Globally lipases are the most attractive source of research, as it has numerous applications in various industries like food industry, paper and pulp industry, preparation of beverages etc. A lipase producing bacterium, Pseudomonas stutzeri, was isolated from sea water. The bacterial culture was introduced to the physical and chemical mutagens and then allowed to grow on the solid media. A number of mutated clones were produced which were further followed by examining their lipase activity. There was a significant increase in the extracellular lipase activity i.e. 13, 56 and 14 folds increase in the case of UV mutation, sodium azide, and NTG respectively. Further, the mutants were subcultured and stability was observed in NTG mutants. The lipase production from the NTG mutants was optimized using Response Surface Methodology (RSM). The maximum lipase activity of 1132.6 U/ml was obtained which was about 7 folds higher than the parent strain using the process which utilized the residual coconut cake, a byprtoduct of coconut oil industries and the sea water which makes the process sustainable.
AB - Globally lipases are the most attractive source of research, as it has numerous applications in various industries like food industry, paper and pulp industry, preparation of beverages etc. A lipase producing bacterium, Pseudomonas stutzeri, was isolated from sea water. The bacterial culture was introduced to the physical and chemical mutagens and then allowed to grow on the solid media. A number of mutated clones were produced which were further followed by examining their lipase activity. There was a significant increase in the extracellular lipase activity i.e. 13, 56 and 14 folds increase in the case of UV mutation, sodium azide, and NTG respectively. Further, the mutants were subcultured and stability was observed in NTG mutants. The lipase production from the NTG mutants was optimized using Response Surface Methodology (RSM). The maximum lipase activity of 1132.6 U/ml was obtained which was about 7 folds higher than the parent strain using the process which utilized the residual coconut cake, a byprtoduct of coconut oil industries and the sea water which makes the process sustainable.
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M3 - Conference contribution
AN - SCOPUS:85117903163
T3 - Technologies for Sustainable Development - Proceedings of the 7th Nirma University International Conference on Engineering, NUICONE 2019
SP - 42
EP - 47
BT - Technologies for Sustainable Development - Proceedings of the 7th Nirma University International Conference on Engineering, NUICONE 2019
A2 - Mahajan, Alka
A2 - Patel, Parul
A2 - Sharma, Priyanka
PB - CRC Press/Balkema
T2 - 7th Nirma University International Conference on Engineering: Technologies for Sustainable Development, NUICONE 2019
Y2 - 21 November 2019 through 22 November 2019
ER -