TY - JOUR
T1 - Activity guided isolation of antioxidant tannoid principles from Anogeissus latifolia
AU - Govindarajan, R.
AU - Vijayakumar, M.
AU - Shirwaikar, A.
AU - Rawat, A.K.S.
AU - Mehrotra, S.
AU - Pushpangadan, P.
N1 - Cited By :6
Export Date: 10 November 2017
CODEN: NPSCF
Correspondence Address: Govindarajan, R.; Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow-226 001, India; email: govind108@yahoo.com
Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; acetic acid ethyl ester, 141-78-6; butanol, 35296-72-1, 71-36-3; ellagic acid, 476-66-4; gallic acid, 149-91-7; silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; xanthine oxidase, 9002-17-9
References: Apparanantham, T., Chelladurai, V., Glimpses of folk medicines of Dharmapuri forest division, Tamil Nadu (1986) Anc. Sci. Life., 5, pp. 182-185; Balla, N.P., Sahu, T.R., Mishra, G.P., Traditional plant medicines of Sagar district, Madhya Pradhesh (1982) J. Econ. Taxon. Bot., 3, pp. 23-32; Barch, D.H., Rundhaugen, L.M., Stoner, G.D., Pillay, N.S., Rosche, W.A., Structure function relationships of the dietary anticarcinogen ellagic acid (1996) Carcinogenisis, 17, pp. 265-269; Deshpande, V.H., Patil, A.D., Rama Rao, A.V., Venkatraman, K., 3,3′-Di-O-methylellagic acid-4′-β-D-xyloside and 3,4,3′-tri-O-methylflavellagic acid-4′-β-D-glucoside from Anogeissus latifolia bark (1976) Indian J. Chem., 14 B, pp. 641-643; Govindarajan, R., Vijayakumar, M., Rao, Ch.V., Rawat, A.K.S., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Antioxidant potential of Anogeissus latifolia (2004) Biol. Pharm. Bull., 27, pp. 1266-1269; Govindarajan, R., Vijayakumar, M., Rao, Ch.V., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Healing potential of Anogeissus latifolia in dermal wounds (2004) Acta Pharm., 54, pp. 331-338; Gyamfi, M.A., Aniya, Y., Antioxidant properties of Thonningianin A, isolated from the African medicinal herbs, Thonningia sanguinea (2002) Biochem. Pharmacol., 63, pp. 1725-1737; Jain, S.K., Tarafder, C.R., Medicinal plant lore of the Santal. A review of P.O. bedding's work (1970) Econ. Bot., 24, pp. 241-278; Mishra, R., Billore, K.V., Some ethnobotanical lores from Banswara district, Kerala state (1983) Nagarjun, 26, pp. 229-231; Nawwar, M.A.M., Souleman, A.M.A., 3,4,8,9,10-Pentahydroxy-dibenzo[b,d]pyran-6-one from Tamarix nilotica (1984) Phytochemistry, 23, p. 2966; Peach, K., Tracy, M.V., (1955) Modern Methods of Analysis, , Heidelberg, Springer Verlag; Ramachandran, V.S., Nair, N.C., Ethnobotanical studies in Cannanore disctrict, Kerala state (1981) J. Econ. Taxon. Bot., 2, pp. 183-190; Reddy, K.K., Rajadurai, S., Nayudamma, Y., Studies on Dhava (Anogiessus latifolia) tannins: Part III-polyphenols of bark, sapwood and heartwood of Dhava (1965) Indian J. Chem., 3, pp. 308-310; Roy, G.P., Chaturvedi, D.P., Ethnomedicinal trees of Abujh-Marh area, Madhya Pradesh (1986) Folklore, 27, pp. 95-100; Scalbert, A., Monties, B., Favre, J.M., Polyphenols of Quercus robur. adult tree and in votro grown calli and shoots (1988) Phytochemistry, 27, pp. 3483-3488; Schmeda, H.G., Gutierrez, M.I., Loyola, J.I., Zuniga, J., Biological activity and xanthine oxidase inhibitors from Scirpus califormicus (C.A. Mey.) Steud (1996) Phytother. Res., 10, pp. 683-685; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcumnoids (1996) Drug Res., 46, pp. 169-171; Taylor, W.H., Sinha, A., Khan, I.A., McDaniel, S.T., Esko, J.D., Primers of glycosaminoglycan biosynthesis from Peruvian rain forest plants (1998) J. Biol. Chem., 273, pp. 22260-22266; Theismania, K.C., Kuttan, R., Inhibition of liver fibrosis by ellagic acid (1996) Indian J. Physiol. Pharmacol., 40, pp. 363-366
PY - 2005
Y1 - 2005
N2 - Oxidative stress is an important causative factor in several human chronic diseases, such as atherosclerosis, cardiovascular disorders, mutagenesis, cancer, several neurodegenerative disorders, and the aging process. Phenolics and tannins are reported to be good antioxidants. Anogeissus latifolia (Combretaceae) bark has been used in the Indian traditional systems of medicine for curing a variety of ailments, but scientific validation is not available till date. Hence the present study was undertaken to isolate antioxidant compounds by activity-guided isolation. Inhibtion of diphenyl picryl hydrazyl (DPPH) and Xanthine oxidase along with photochemiluminescence assay were used as bioassay for antioxidant activity. Activity guided isolation was carried out using silica column and the compounds were quantified using HPLC. Ethyl acetate and butanol fraction exhibited potent antioxidant activity. Bioassay-guided isolation led to isolation of ellagic acid (1) and dimethyl ellagic acid (2) as the main active compounds, which along with gallic acid were quantified by HPLC. Thus we conclude that these three major tannoid principles present in A. latifolia, are responsible for the antioxidant potential and possibly their therapeutic potential.
AB - Oxidative stress is an important causative factor in several human chronic diseases, such as atherosclerosis, cardiovascular disorders, mutagenesis, cancer, several neurodegenerative disorders, and the aging process. Phenolics and tannins are reported to be good antioxidants. Anogeissus latifolia (Combretaceae) bark has been used in the Indian traditional systems of medicine for curing a variety of ailments, but scientific validation is not available till date. Hence the present study was undertaken to isolate antioxidant compounds by activity-guided isolation. Inhibtion of diphenyl picryl hydrazyl (DPPH) and Xanthine oxidase along with photochemiluminescence assay were used as bioassay for antioxidant activity. Activity guided isolation was carried out using silica column and the compounds were quantified using HPLC. Ethyl acetate and butanol fraction exhibited potent antioxidant activity. Bioassay-guided isolation led to isolation of ellagic acid (1) and dimethyl ellagic acid (2) as the main active compounds, which along with gallic acid were quantified by HPLC. Thus we conclude that these three major tannoid principles present in A. latifolia, are responsible for the antioxidant potential and possibly their therapeutic potential.
M3 - Article
SN - 1226-3907
VL - 11
SP - 174
EP - 178
JO - Natural Product Sciences
JF - Natural Product Sciences
IS - 3
ER -
