Acute toxicity studies and determination of median lethal dose

J. Shetty Akhila, Shyamjith, Deepa, M. C. Alwar

Research output: Contribution to journalReview articlepeer-review

178 Citations (Scopus)


Whenever an investigator administers a chemical substance to a biological system, different types of interactions can occur and a series of dose-related responses result. In most cases these responses are desired and useful, but there are a number of other effects which are not advantageous. These may or may not be harmful to the patients. The types of toxicity tests which are routinely performed by pharmaceutical manufactures in the investigation of a new drug involve acute, sub-acute and chronic toxicity. Acute toxicity is involved in estimation of LD50 (the dose which has proved to be lethal (causing death) to 50% of the tested group of animals). Determination of acute oral toxicity is usually an initial screening step in the assessment and evaluation of the toxic characteristics of all compounds. This article reviews the methods so far utilized for the determination of median lethal dose (LD50) and the new changes which could be made. This has to go through the entire process of validation with different categories of substances before its final acceptance by regulatory bodies.

Original languageEnglish
Pages (from-to)917-920
Number of pages4
JournalCurrent Science
Issue number7
Publication statusPublished - 10-10-2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General
  • Agricultural and Biological Sciences(all)


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