Adaptive response to low dose of EMS or MMS in human peripheral blood lymphocytes

S. K. Harish, K. P. Guruprasad, Riaz Mahmood, V. Vasudev, K. R. Manjunath, G. K. Chethan

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9 Citations (SciVal)


Human peripheral blood lymphocytes stimulated in vitro for 6 hr were exposed to a low (conditioning) dose of ethyl methanesulfonate (EMS; 1.5×10-4 M) or methyl methanesulfonate (MMS; 1.5×10-5 M). After 6 hr, the cells were treated with a high (challenging) concentration of the same agent (1.5×10-3 M EMS or 1.5×10-4 M MMS). The cells that received both conditioning and challenging doses became less sensitive to the induction of sister chromatid exchanges (SCEs) than those which did not receive the pretreatment with EMS or MMS. They responded with lower frequencies of SCEs. This suggests that conditioning dose of EMS or MMS has offered the lymphocytes to have decreased SCEs. This led to the realization that pre-exposure of lymphocytes to low dose can cause the induction of repair activity. This is a clear indication of the existence of adaptive response induced by alkylating agents whether it is ethylating or methylating in human lymphocytes in vitro.

Original languageEnglish
Pages (from-to)1147-1150
Number of pages4
JournalIndian Journal of Experimental Biology
Issue number11
Publication statusPublished - 11-1998

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Medicine(all)
  • Molecular Biology
  • Cell Biology


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