TY - JOUR
T1 - Ameliorative potential of Operculina turpethum against streptozotocin-induced diabetes in rats
T2 - biochemical and histopathological studies
AU - Choudhary, Neeraj
AU - Khatik, Gopal L.
AU - Sharma, Rekha
AU - Khurana, Navneet
AU - Lobo, Richard
AU - Bhatt, Shvetank
AU - Tewari, Devesh
AU - Suttee, Ashish
N1 - Funding Information:
We thank the management of Lovely Professional University, Phagwara, for providing the necessary support and research facility for conducting the research work. We express our gratitude towards Prof Dr. Kuldeep Gupta, Department of Veterinary Pathology, Guru Angad Dev University, and Ludhiana for interpretation of histopathological slides and Dr. M.P Singh National Laboratories, Phagwara for providing support in biochemical and hematology analysis.
Publisher Copyright:
© 2021, King Abdulaziz City for Science and Technology.
PY - 2021/6
Y1 - 2021/6
N2 - The study aimed to investigate the acute toxicity, antidiabetic potential (in-vitro and in-vivo) of the Operculina turpethum (L.) Silva Manso at fraction level. The plant was fractionated into different fractions, i.e., flavonoid fraction (OTFF), tannin fraction (OTTF), saponin fraction (OTSF). In-vitro alpha-amylase inhibition assay revealed that OTFF was found to be more potent than standard Acarbose. The plant fractions were evaluated by MTT assay at different concentrations ranging from 100 to 1000 µg/ml. All the fractions were further evaluated for their safety profile, and the biochemical, hematology and histopathology result exhibits that the OTFF fraction produces mild toxicity at organ level at a concentration of 2000 mg/kg in albino mice. The in-vivo antidiabetic study was carried out on Sprague-Dawley rats using high-fat diet (HFD) feeding streptozotocin (STZ) diabetic model, and the biochemical, histopathology research findings represent that OTFF at a concentration of 500 mg/kg, p.o. was found to be highly significant among all the fractions and found to be more potent than the standard Acarbose. LC–MS characterization of the bioactive fraction OTFF showed the presence of rutin with m/z 610.52 in 50.50% and Apigenin 7-O-6'' acetyl-glucoside with m/z 475.42 in 24.10%; from molecular docking study, it is predicted that the fraction primarily acts as an alpha-amylase inhibitor and PPAR gamma agonist. In conclusion, the plant’s OTFF fraction acts as a potential therapeutic agent for Type II diabetes mellitus.
AB - The study aimed to investigate the acute toxicity, antidiabetic potential (in-vitro and in-vivo) of the Operculina turpethum (L.) Silva Manso at fraction level. The plant was fractionated into different fractions, i.e., flavonoid fraction (OTFF), tannin fraction (OTTF), saponin fraction (OTSF). In-vitro alpha-amylase inhibition assay revealed that OTFF was found to be more potent than standard Acarbose. The plant fractions were evaluated by MTT assay at different concentrations ranging from 100 to 1000 µg/ml. All the fractions were further evaluated for their safety profile, and the biochemical, hematology and histopathology result exhibits that the OTFF fraction produces mild toxicity at organ level at a concentration of 2000 mg/kg in albino mice. The in-vivo antidiabetic study was carried out on Sprague-Dawley rats using high-fat diet (HFD) feeding streptozotocin (STZ) diabetic model, and the biochemical, histopathology research findings represent that OTFF at a concentration of 500 mg/kg, p.o. was found to be highly significant among all the fractions and found to be more potent than the standard Acarbose. LC–MS characterization of the bioactive fraction OTFF showed the presence of rutin with m/z 610.52 in 50.50% and Apigenin 7-O-6'' acetyl-glucoside with m/z 475.42 in 24.10%; from molecular docking study, it is predicted that the fraction primarily acts as an alpha-amylase inhibitor and PPAR gamma agonist. In conclusion, the plant’s OTFF fraction acts as a potential therapeutic agent for Type II diabetes mellitus.
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U2 - 10.1007/s13205-021-02811-x
DO - 10.1007/s13205-021-02811-x
M3 - Article
AN - SCOPUS:85107068228
SN - 2190-572X
VL - 11
JO - 3 Biotech
JF - 3 Biotech
IS - 6
M1 - 309
ER -
