TY - JOUR
T1 - Aminolysis of polycaprolactone nanofibers for applications in uterine tissue engineering
AU - Hanuman, Srividya
AU - Pande, Gopal
AU - Nune, Manasa
N1 - Funding Information:
M.N would like to acknowledge Department of Science & Technology for SERB start-up research grant (SRG/2019/002130) for the financial support. M.N and S.H would also like to thank MAHE intramural grant (MAHE/DREG/PHD/IMF/2019) and Dr. T.M.A. Pai Ph.D. Scholarship respectively from Manipal Academy of Higher Education. All authors would also extend thanks to the Manipal Institute of Regenerative Medicine, MAHE for the infrastructural support.
Publisher Copyright:
© (2021) Society for Biomaterials & Artificial Organs #20048621
PY - 2021/10/10
Y1 - 2021/10/10
N2 - One in every five hundred women in their fertile years are affected by Absolute Uterine Factor Infertility (AUFI) whose treatment is usually organ transplantation. But the success rate of complete recovery after this procedure is very minimal and there is also shortage in donor organs and long-term immunosuppression. Therefore, the concepts of tissue engineering in uterus have emerged as a potential solution. Uterine tissue is highly elastic in nature and is made up of several sheets of membranes and elastic fibrils. Therefore, the objective of this study was to create a nanofibrous scaffold by electrospinning method similar to native ECM using polycaprolactone (PCL) as a biomaterial. PCL nanofibers, being highly hydrophobic, were surface modified by aminolysis, where primarily amine groups were introduced. The modified nanofibers were characterized by contact angle analysis; Fourier transformed infrared spectroscopy (FTIR), and ninhydrin assay. For assessing their cytocompatibility, primary human uterine fibroblasts were cultured and analysed for their adhesion, morphology, proliferation, and viability in comparison to unmodified PCL fibers and tissue culture polystyrene (TCPS). Our results showed that immobilization of NH2 groups on the PCL nanofibers significantly improved their capacity for uterine cell attachment and proliferation. The modified PCL fibers also provided the biochemical signals for the uterine cells to acquire a morphology indicating that they could be potentially used as a promising scaffold for uterine tissue engineering applications.
AB - One in every five hundred women in their fertile years are affected by Absolute Uterine Factor Infertility (AUFI) whose treatment is usually organ transplantation. But the success rate of complete recovery after this procedure is very minimal and there is also shortage in donor organs and long-term immunosuppression. Therefore, the concepts of tissue engineering in uterus have emerged as a potential solution. Uterine tissue is highly elastic in nature and is made up of several sheets of membranes and elastic fibrils. Therefore, the objective of this study was to create a nanofibrous scaffold by electrospinning method similar to native ECM using polycaprolactone (PCL) as a biomaterial. PCL nanofibers, being highly hydrophobic, were surface modified by aminolysis, where primarily amine groups were introduced. The modified nanofibers were characterized by contact angle analysis; Fourier transformed infrared spectroscopy (FTIR), and ninhydrin assay. For assessing their cytocompatibility, primary human uterine fibroblasts were cultured and analysed for their adhesion, morphology, proliferation, and viability in comparison to unmodified PCL fibers and tissue culture polystyrene (TCPS). Our results showed that immobilization of NH2 groups on the PCL nanofibers significantly improved their capacity for uterine cell attachment and proliferation. The modified PCL fibers also provided the biochemical signals for the uterine cells to acquire a morphology indicating that they could be potentially used as a promising scaffold for uterine tissue engineering applications.
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M3 - Article
AN - SCOPUS:85119616121
SN - 0971-1198
VL - 35
SP - 347
EP - 353
JO - Trends in Biomaterials and Artificial Organs
JF - Trends in Biomaterials and Artificial Organs
IS - 4
ER -