Abstract
Aim: To develop a new sensitive RP-HPLC method for simultaneous estimation of 5-fluorouracil (5-FU) and sonidegib (SDG). Materials & methods: Analytical and bioanalytical methods for simultaneous quantification of 5-FU and SDG in bulk, nanoformulations and in rat plasma were developed and validated using a gradient elution technique. Results: Separation of the analytes was effected on a Luna® C18 LC column using a mobile mixture comprising acetonitrile and acidified water. 5-FU and SDG were extracted from plasma matrix using liquid-liquid extraction. The applicability of the method was verified through single-dose oral pharmacokinetic study in Wistar rats. Conclusion: The developed methods allow a specific, sensitive and steady analytical procedure for the simultaneous estimation of 5-FU and SDG in nanoformulations and biological matrix.
Original language | English |
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Pages (from-to) | 29-45 |
Number of pages | 17 |
Journal | Bioanalysis |
Volume | 14 |
Issue number | 1 |
DOIs | |
Publication status | Published - 01-2022 |
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Pharmacology, Toxicology and Pharmaceutics(all)
- Clinical Biochemistry
- Medical Laboratory Technology