Analytical and bioanalytical HPLC method for simultaneous estimation of 5-fluorouracil and sonidegib

Bharath S. Padya; Aswathi R. Hegde; Sadhana P. Mutalik; Swati Biswas; Srinivas Mutalik

doi:10.4155/bio-2021-0212

Analytical and bioanalytical HPLC method for simultaneous estimation of 5-fluorouracil and sonidegib

Bharath S. Padya
, Aswathi R. Hegde
, Sadhana P. Mutalik
, Swati Biswas
, Srinivas Mutalik^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Aim: To develop a new sensitive RP-HPLC method for simultaneous estimation of 5-fluorouracil (5-FU) and sonidegib (SDG). Materials & methods: Analytical and bioanalytical methods for simultaneous quantification of 5-FU and SDG in bulk, nanoformulations and in rat plasma were developed and validated using a gradient elution technique. Results: Separation of the analytes was effected on a Luna® C18 LC column using a mobile mixture comprising acetonitrile and acidified water. 5-FU and SDG were extracted from plasma matrix using liquid-liquid extraction. The applicability of the method was verified through single-dose oral pharmacokinetic study in Wistar rats. Conclusion: The developed methods allow a specific, sensitive and steady analytical procedure for the simultaneous estimation of 5-FU and SDG in nanoformulations and biological matrix.

Original language	English
Pages (from-to)	29-45
Number of pages	17
Journal	Bioanalysis
Volume	14
Issue number	1
DOIs	https://doi.org/10.4155/bio-2021-0212
Publication status	Published - 01-2022

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Clinical Biochemistry
Medical Laboratory Technology

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@article{20b9364547e448d5a7ffbdef80088c52,

title = "Analytical and bioanalytical HPLC method for simultaneous estimation of 5-fluorouracil and sonidegib",

abstract = "Aim: To develop a new sensitive RP-HPLC method for simultaneous estimation of 5-fluorouracil (5-FU) and sonidegib (SDG). Materials \& methods: Analytical and bioanalytical methods for simultaneous quantification of 5-FU and SDG in bulk, nanoformulations and in rat plasma were developed and validated using a gradient elution technique. Results: Separation of the analytes was effected on a Luna{\textregistered} C18 LC column using a mobile mixture comprising acetonitrile and acidified water. 5-FU and SDG were extracted from plasma matrix using liquid-liquid extraction. The applicability of the method was verified through single-dose oral pharmacokinetic study in Wistar rats. Conclusion: The developed methods allow a specific, sensitive and steady analytical procedure for the simultaneous estimation of 5-FU and SDG in nanoformulations and biological matrix. ",

author = "Padya, \{Bharath S.\} and Hegde, \{Aswathi R.\} and Mutalik, \{Sadhana P.\} and Swati Biswas and Srinivas Mutalik",

note = "Funding Information: The authors are thankful to: All India Council for Technical Education (AICTE), Government of India, New Delhi for providing National Doctoral Fellowship to Bharath Singh Padya; and Department of Science and Technology (DST), Government of India, New Delhi for providing DST-INSPIRE Fellowship to Sadhana P Mutalik. Funding Information: The authors also thank Manipal College of Pharmaceutical Sciences and Manipal Academy of Higher Education, Manipal for providing financial support and research facilities. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: {\textcopyright} 2021 Newlands Press.",

year = "2022",

month = jan,

doi = "10.4155/bio-2021-0212",

language = "English",

volume = "14",

pages = "29--45",

journal = "Bioanalysis",

issn = "1757-6180",

publisher = "Taylor and Francis Ltd.",

number = "1",

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TY - JOUR

T1 - Analytical and bioanalytical HPLC method for simultaneous estimation of 5-fluorouracil and sonidegib

AU - Padya, Bharath S.

AU - Hegde, Aswathi R.

AU - Mutalik, Sadhana P.

AU - Biswas, Swati

AU - Mutalik, Srinivas

N1 - Funding Information: The authors are thankful to: All India Council for Technical Education (AICTE), Government of India, New Delhi for providing National Doctoral Fellowship to Bharath Singh Padya; and Department of Science and Technology (DST), Government of India, New Delhi for providing DST-INSPIRE Fellowship to Sadhana P Mutalik. Funding Information: The authors also thank Manipal College of Pharmaceutical Sciences and Manipal Academy of Higher Education, Manipal for providing financial support and research facilities. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: © 2021 Newlands Press.

PY - 2022/1

Y1 - 2022/1

N2 - Aim: To develop a new sensitive RP-HPLC method for simultaneous estimation of 5-fluorouracil (5-FU) and sonidegib (SDG). Materials & methods: Analytical and bioanalytical methods for simultaneous quantification of 5-FU and SDG in bulk, nanoformulations and in rat plasma were developed and validated using a gradient elution technique. Results: Separation of the analytes was effected on a Luna® C18 LC column using a mobile mixture comprising acetonitrile and acidified water. 5-FU and SDG were extracted from plasma matrix using liquid-liquid extraction. The applicability of the method was verified through single-dose oral pharmacokinetic study in Wistar rats. Conclusion: The developed methods allow a specific, sensitive and steady analytical procedure for the simultaneous estimation of 5-FU and SDG in nanoformulations and biological matrix.

AB - Aim: To develop a new sensitive RP-HPLC method for simultaneous estimation of 5-fluorouracil (5-FU) and sonidegib (SDG). Materials & methods: Analytical and bioanalytical methods for simultaneous quantification of 5-FU and SDG in bulk, nanoformulations and in rat plasma were developed and validated using a gradient elution technique. Results: Separation of the analytes was effected on a Luna® C18 LC column using a mobile mixture comprising acetonitrile and acidified water. 5-FU and SDG were extracted from plasma matrix using liquid-liquid extraction. The applicability of the method was verified through single-dose oral pharmacokinetic study in Wistar rats. Conclusion: The developed methods allow a specific, sensitive and steady analytical procedure for the simultaneous estimation of 5-FU and SDG in nanoformulations and biological matrix.

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U2 - 10.4155/bio-2021-0212

DO - 10.4155/bio-2021-0212

M3 - Article

AN - SCOPUS:85120630137

SN - 1757-6180

VL - 14

SP - 29

EP - 45

JO - Bioanalysis

JF - Bioanalysis

IS - 1

ER -

Analytical and bioanalytical HPLC method for simultaneous estimation of 5-fluorouracil and sonidegib

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