Analytical method development and validation for aspirin

S. Suresh Kumar; Latif D. Jamadar; Krishnamurthy Bhat; Prashant B. Musmade; S. G. Vasantharaju; N. Udupa

Analytical method development and validation for aspirin

S. Suresh Kumar, Latif D. Jamadar, Krishnamurthy Bhat, Prashant B. Musmade, S. G. Vasantharaju, N. Udupa

Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal
Department of Pharmacy Management, Manipal College of Pharmaceutical Sciences, Manipal

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for aspirin in presence of its degradation products is developed and validated. The method employed Hypersil BDS C ₁₈ (100 x 4.6 mm 5μ) column as stationary phase.The mobile phase consisted of sodium perchlorate buffer (pH 2.5): acetonitrile: isopropyl alcohol (85:14:1 % v/v). It is pumped through the chromatographic system at a flow rate of 1.5 ml min^-1.The UV detector is operated at 275 nm. This system was found to give good resolution between aspirin and its degradation products. Method was validated as per ICH guidelines.

Original language	English
Pages (from-to)	389-399
Number of pages	11
Journal	International Journal of ChemTech Research
Volume	2
Issue number	1
Publication status	Published - 01-01-2010

All Science Journal Classification (ASJC) codes

General Chemistry
General Chemical Engineering

Cite this

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abstract = "A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for aspirin in presence of its degradation products is developed and validated. The method employed Hypersil BDS C 18 (100 x 4.6 mm 5μ) column as stationary phase.The mobile phase consisted of sodium perchlorate buffer (pH 2.5): acetonitrile: isopropyl alcohol (85:14:1 % v/v). It is pumped through the chromatographic system at a flow rate of 1.5 ml min-1.The UV detector is operated at 275 nm. This system was found to give good resolution between aspirin and its degradation products. Method was validated as per ICH guidelines.",

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AU - Bhat, Krishnamurthy

AU - Musmade, Prashant B.

AU - Vasantharaju, S. G.

AU - Udupa, N.

PY - 2010/1/1

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N2 - A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for aspirin in presence of its degradation products is developed and validated. The method employed Hypersil BDS C 18 (100 x 4.6 mm 5μ) column as stationary phase.The mobile phase consisted of sodium perchlorate buffer (pH 2.5): acetonitrile: isopropyl alcohol (85:14:1 % v/v). It is pumped through the chromatographic system at a flow rate of 1.5 ml min-1.The UV detector is operated at 275 nm. This system was found to give good resolution between aspirin and its degradation products. Method was validated as per ICH guidelines.

AB - A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for aspirin in presence of its degradation products is developed and validated. The method employed Hypersil BDS C 18 (100 x 4.6 mm 5μ) column as stationary phase.The mobile phase consisted of sodium perchlorate buffer (pH 2.5): acetonitrile: isopropyl alcohol (85:14:1 % v/v). It is pumped through the chromatographic system at a flow rate of 1.5 ml min-1.The UV detector is operated at 275 nm. This system was found to give good resolution between aspirin and its degradation products. Method was validated as per ICH guidelines.

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Analytical method development and validation for aspirin

Abstract

All Science Journal Classification (ASJC) codes

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