TY - JOUR
T1 - Analytical Method Development and Validation for HPLC-ECD Determination of Moxifloxacin in Marketed Formulations
AU - Phani Sekhar Reddy, G.
AU - Navyasree, K. S.
AU - Jagadish, P. C.
AU - Bhat, Krishnamurthy
N1 - Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - An HPLC-ECD analytical method with high reproducibility and wide linearity range has been developed and validated. Moxifloxacin was separated and identified using this method with a simple mobile phase comprising Britton Robinson buffer pH 5.0 and methanol (93: 7 v/v) flowing at a rate of 0.5 mL/min through Acclaim C18 column (150 mm × 4.6 mm × 5 im) maintained at 35°C and detected at redox potential value of 1.0 V. The LOD and LOQ were found to be 2.2 and 6.6 μg/mL, respectively. The HPLC-ECD method of moxifloxacin analysis was validated as per ICH Q2R1 guidelines and applied for assay of marketed moxifloxacin formulations to establish the acceptable recovery.
AB - An HPLC-ECD analytical method with high reproducibility and wide linearity range has been developed and validated. Moxifloxacin was separated and identified using this method with a simple mobile phase comprising Britton Robinson buffer pH 5.0 and methanol (93: 7 v/v) flowing at a rate of 0.5 mL/min through Acclaim C18 column (150 mm × 4.6 mm × 5 im) maintained at 35°C and detected at redox potential value of 1.0 V. The LOD and LOQ were found to be 2.2 and 6.6 μg/mL, respectively. The HPLC-ECD method of moxifloxacin analysis was validated as per ICH Q2R1 guidelines and applied for assay of marketed moxifloxacin formulations to establish the acceptable recovery.
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U2 - 10.1007/s11094-018-1879-1
DO - 10.1007/s11094-018-1879-1
M3 - Article
AN - SCOPUS:85056115110
SN - 0091-150X
VL - 52
SP - 674
EP - 679
JO - Pharmaceutical Chemistry Journal
JF - Pharmaceutical Chemistry Journal
IS - 7
ER -