Analytical Method Development and Validation of Derivatized Inulin and its application to Herbal formulation by Simple UV Spectroscopy

Inulin is a high molecular weight carbohydrate made up of fructose molecules attached with glucosyl moieties in the end. Due to its high molecular weight direct analysis is tedious hence we opted for derivatization. Inulin was derivatized using Seliwanoff’s reagent and was analyzed using UV-spectrophotometry. This method was validated for specificity, linearity, precision, accuracy, the limit of detection (LOD), and the limit of quantitation (LOQ). It was seen that all the parameters met the acceptance criteria. After validation the method was applied for the formulation and %recovery was determined.