TY - JOUR
T1 - Analytical Method Development and Validation of Derivatized Inulin and its application to Herbal formulation by Simple UV Spectroscopy
AU - Kanabaragi, Gouri V.
AU - Vasantharaju, S. G.
AU - Muddukrishna, B. S.
AU - Syeda, Alisha
AU - Gaddam, Niharika
N1 - Publisher Copyright:
© RJPT All right reserved.
PY - 2023/9
Y1 - 2023/9
N2 - Inulin is a high molecular weight carbohydrate made up of fructose molecules attached with glucosyl moieties in the end. Due to its high molecular weight direct analysis is tedious hence we opted for derivatization. Inulin was derivatized using Seliwanoff’s reagent and was analyzed using UV-spectrophotometry. This method was validated for specificity, linearity, precision, accuracy, the limit of detection (LOD), and the limit of quantitation (LOQ). It was seen that all the parameters met the acceptance criteria. After validation the method was applied for the formulation and %recovery was determined.
AB - Inulin is a high molecular weight carbohydrate made up of fructose molecules attached with glucosyl moieties in the end. Due to its high molecular weight direct analysis is tedious hence we opted for derivatization. Inulin was derivatized using Seliwanoff’s reagent and was analyzed using UV-spectrophotometry. This method was validated for specificity, linearity, precision, accuracy, the limit of detection (LOD), and the limit of quantitation (LOQ). It was seen that all the parameters met the acceptance criteria. After validation the method was applied for the formulation and %recovery was determined.
UR - https://www.scopus.com/pages/publications/85178952640
UR - https://www.scopus.com/inward/citedby.url?scp=85178952640&partnerID=8YFLogxK
U2 - 10.52711/0974-360X.2023.00708
DO - 10.52711/0974-360X.2023.00708
M3 - Article
AN - SCOPUS:85178952640
SN - 0974-3618
VL - 16
SP - 4325
EP - 4328
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 9
ER -