Antioxidant activity of Desmodium gangeticum and its phenolics in arthritic rats

R. Govindarajan, M. Vijayakumar, A. Shirwaikar, A.K.S. Rawat, S. Mehrotra, P. Pushpangadan

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Total alcoholic extract of Desmodium gangeticum, which exhibited significant anti-inflammatory activity, was evaluated for the possible mode of action by studying its antioxidant potential in adjuvant-induced arthritic rats. Activity guided fractionation and isolation were carried out. The phenolics fraction showed maximum potency. Solid phase extraction followed by preparative HPLC of the active phenolic fraction yielded for the first time two potent antioxidant compounds, caffeic acid and chlorogenic acid, from this plant. The biological antioxidant defense system, involving superoxide dismutase, glutathione and catalase, showed a significant increase with their levels close to the normal control with a decrease in the lipid peroxide content upon administration of D. gangeticum extract (100 mg kg-1) and its phenolics (50 mg kg-1) in arthritic rats, thereby indicating the extracts antioxidant property under arthritic conditions.
Original languageEnglish
Pages (from-to)489-496
Number of pages8
JournalActa Pharmaceutica
Volume56
Issue number4
Publication statusPublished - 2006
Externally publishedYes

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