TY - JOUR
T1 - Antioxidant activity of Desmodium gangeticum and its phenolics in arthritic rats
AU - Govindarajan, R.
AU - Vijayakumar, M.
AU - Shirwaikar, A.
AU - Rawat, A.K.S.
AU - Mehrotra, S.
AU - Pushpangadan, P.
N1 - Cited By :16
Export Date: 10 November 2017
CODEN: ACPHE
Correspondence Address: Shirwaikar, A.; Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow-226 001, India; email: [email protected]
Chemicals/CAS: caffeic acid, 27323-69-9, 331-39-5; catalase, 9001-05-2; chlorogenic acid, 327-97-9; glutathione, 70-18-8; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Antioxidants; Catalase, 1.11.1.6; Glutathione, 70-18-8; Phenols; Plant Extracts; Superoxide Dismutase, 1.15.1.1; Thiobarbituric Acid Reactive Substances
Manufacturers: Sigma, United States
References: Krane, S.M., Conca, W., Stephenson, M.L., Amento, E.P., Goldring, M.B., Mechanisms of matrix degradation in rheumatoid arthritis (1990) Ann. New York Acad. Sci., 580, pp. 340-354; Gutteridge, J.M.C., Antioxidant properties of the proteins ceruloplasmin, albumin and transferrin A. A study of their activity in serum and synovial fluid from patients with rheumatoid arthritis (1986) Biochem. Biophys. Acta, 869, pp. 119-127; Aruoma, O.I., Characterization of drugs as antioxidant prophylactics (1996) Free Rad. Biol. Med., 20, pp. 675-705; Holley, A.E., Cheeseman, K.H., Measuring free radical reactions in vivo (1993) Br. Med. Bull., 49, pp. 494-505; Chopra, R.N., Nayar, S.L., Chopra, I.C., (1956) Glossary of Indian Medicinal Plants, , CSIR, New Delhi; Ghosh, D., Anandkumar, A., Anti-inflammatory and analgesic activities of gangetin - A pterocarpenoid from Desmodium gangeticum (1981) Indian J. Pharmacol., 15, pp. 391-402; Purushothman, K.K., Kishore, V.M., Narayanaswamy, V., The structure and stereochemistry of gangetin. A new pterocarpan from Desmodium gangeticum (1971) J. Chem. Soc., pp. 2420-2422; Ghosal, S., Bhattacharya, S.K., Desmodium alkaloids II, Chemical and pharmacological evaluation of D. gangeticum (1972) Planta Med., 22, pp. 434-440; Govindarajan, R., Rastogi, S., Vijayakumar, M., Rawat, A.K.S., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Studies on antioxidant activities of Desmodium gangeticum (2003) Biol. Pharm. Bull., 26, pp. 1424-1427; Al-Qirim, T.M., Shahwan, M., Zaidi, K.R., Uddin, Q., Banu, N., Effect of khat, its constituents and restraint stress in free radical metabolism of rats (2002) J. Ethnopharmacol., 83, pp. 245-250; Williams, A.S., Camilleir, J.P., Williams, B.D., Suppression of adjuvant induced by liposomally conjugated methotrexate in the rat (1994) Br. J. Rheumat., 33, pp. 530-533; Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.I., Protein determination using Folin-Ciocalteau reagent (1951) J. Biol. Chem., 193, pp. 438-448; Okhawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal. Biochem., 95, pp. 351-358; Moran, D., De Buitrago, J.M.G., Fernandez, E., Galan, A.I., Munoz, M.E., Jimenez, R., Inhibition of biliary glutathione secretion by cyclosporine A in the rat: Possible mechanisms and role in the cholestasis induced by the drug (1998) J. Hepatol., 29, pp. 68-77; Kakkar, P., Das, B., Viswanathan, P.N., A modified spectrophotometric assay of superoxide dismutase (1984) Indian J. Biochem. Biophys., 21, pp. 130-132; Cohen, G., Dembiec, D., Marcus, J., Measurement of catalase activity in tissue extracts (1970) Anal. Biochem., 34, pp. 30-38; Orsolic, N., Knezevic, A.H., Sver, L., Terzic, S., Basic, I., Immunomodulatory and antimetastatic action of propolis and related polyphenolic compounds (2004) J. Ethnopharmacol., 94, pp. 307-315; Park, J.H., Lee, J.K., Kim, H.S., Chung, S.T., Eom, J.H., Kim, K.A., Chung, S.J., Oh, H.Y., Immunomodulatory effect of caffeic acid phenethyl ester in Balb/c mice (2004) Int. Immunopharmacol., 4, pp. 429-436; Acqua, S.D., Innocenti, G., Antioxidant compounds from Chaerophyllum hirsutum extracts (2004) Fitoterapia, 75, pp. 592-595; McDonald, S., Prenzler, P.D., Antolovich, M., Robards, K., Phenolic content and antioxidant activity of olive extracts (2001) Food Chem., 73, pp. 73-84; Shinmoto, H., Dosako, S., Nakajima, I., Antioxidant activity of bovine lactoferrin on iron/ascorbate induced lipid peroxidation (1992) Biosci. Biotech. Bioch., 56, pp. 2079-2080; Halliwell, B., Free radicals, antioxidants and human disease. Where are we now? (1992) J. Lab. Clin. Med., 119, p. 598
PY - 2006
Y1 - 2006
N2 - Total alcoholic extract of Desmodium gangeticum, which exhibited significant anti-inflammatory activity, was evaluated for the possible mode of action by studying its antioxidant potential in adjuvant-induced arthritic rats. Activity guided fractionation and isolation were carried out. The phenolics fraction showed maximum potency. Solid phase extraction followed by preparative HPLC of the active phenolic fraction yielded for the first time two potent antioxidant compounds, caffeic acid and chlorogenic acid, from this plant. The biological antioxidant defense system, involving superoxide dismutase, glutathione and catalase, showed a significant increase with their levels close to the normal control with a decrease in the lipid peroxide content upon administration of D. gangeticum extract (100 mg kg-1) and its phenolics (50 mg kg-1) in arthritic rats, thereby indicating the extracts antioxidant property under arthritic conditions.
AB - Total alcoholic extract of Desmodium gangeticum, which exhibited significant anti-inflammatory activity, was evaluated for the possible mode of action by studying its antioxidant potential in adjuvant-induced arthritic rats. Activity guided fractionation and isolation were carried out. The phenolics fraction showed maximum potency. Solid phase extraction followed by preparative HPLC of the active phenolic fraction yielded for the first time two potent antioxidant compounds, caffeic acid and chlorogenic acid, from this plant. The biological antioxidant defense system, involving superoxide dismutase, glutathione and catalase, showed a significant increase with their levels close to the normal control with a decrease in the lipid peroxide content upon administration of D. gangeticum extract (100 mg kg-1) and its phenolics (50 mg kg-1) in arthritic rats, thereby indicating the extracts antioxidant property under arthritic conditions.
M3 - Article
SN - 1330-0075
VL - 56
SP - 489
EP - 496
JO - Acta Pharmaceutica
JF - Acta Pharmaceutica
IS - 4
ER -