Applications of 'TissueQuant'- A color intensity quantification tool for medical research

Keerthana Prasad, Bhagath Kumar P., Marx Chakravarthy, Gopalakrishna Prabhu

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


This paper demonstrates the use of TissueQuant - an image analysis tool for quantification of color intensities which was developed for use in medical research where the stained biological specimen such as tissue or antigen needs to be quantified. TissueQuant provides facilities for user interaction to choose and quantify the color of interest and its shades. Gaussian weighting functions are used to provide a color score which quantifies how close the shade is to the user specified reference color. We describe two studies in medical research which use TissueQuant for quantification. The first study evaluated the effect of petroleum-ether extract of Cissus quadrangularis (CQ) on osteoporotic rats. It was found that the analysis results correlated well with the manual evaluation, p< 0.001. The second study evaluated the nerve morphometry and it was found that the adipose and non adipose tissue content was maximum in radial nerve among the five nerves studied.

Original languageEnglish
Pages (from-to)27-36
Number of pages10
JournalComputer Methods and Programs in Biomedicine
Issue number1
Publication statusPublished - 04-2012

All Science Journal Classification (ASJC) codes

  • Software
  • Computer Science Applications
  • Health Informatics


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