Artificial Activation of Murine Oocytes Using Strontium to Derive Haploid and Diploid Parthenotes

Daphne Norma Crasta; Satish Kumar Adiga; Nagarajan Kannan; Guruprasad Kalthur

doi:10.1007/978-1-0716-1979-7_2

Artificial Activation of Murine Oocytes Using Strontium to Derive Haploid and Diploid Parthenotes

Daphne Norma Crasta, Satish Kumar Adiga, Nagarajan Kannan, Guruprasad Kalthur^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Citations (Scopus)

Abstract

Parthenogenesis is a common reproductive strategy among lower animals that involves the development of an embryo from an oocyte, without any contribution from spermatozoon. This phenomenon does not occur naturally in placental mammals. However, the mammalian oocytes can be artificially activated in vitro using mechanical, electrical, and chemical stimuli which can develop up to the blastocyst stage. In this chapter, we describe the protocol for generating haploid and diploid parthenotes from mouse oocytes using strontium as the activating agent under in vitro conditions.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	15-26
Number of pages	12
Volume	2429
DOIs	https://doi.org/10.1007/978-1-0716-1979-7_2
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2429
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

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10.1007/978-1-0716-1979-7_2

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abstract = "Parthenogenesis is a common reproductive strategy among lower animals that involves the development of an embryo from an oocyte, without any contribution from spermatozoon. This phenomenon does not occur naturally in placental mammals. However, the mammalian oocytes can be artificially activated in vitro using mechanical, electrical, and chemical stimuli which can develop up to the blastocyst stage. In this chapter, we describe the protocol for generating haploid and diploid parthenotes from mouse oocytes using strontium as the activating agent under in vitro conditions.",

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AU - Kalthur, Guruprasad

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Artificial Activation of Murine Oocytes Using Strontium to Derive Haploid and Diploid Parthenotes

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