Ascorbate-dependent formation of hydroxyl radicals in the presence of iron chelates.

H. R. Prabhu, S. Krishnamurthy

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12 Citations (Scopus)


The autoxidation of L-ascorbate on incubation in saline phosphate buffer (pH 7.4) is accompanied by hydroxyl radical (.OH) generation. The metal chelator EDTA showed significant inhibition of ascorbate autoxidation and ascorbate-dependent .OH release. On the other hand, Fe2+ (EDTA) greatly augmented both ascorbate autoxidation and ascorbate-dependent .OH production. The biological iron chelating compounds such as ATP, ADP, citrate and pyrophosphate suppressed both ascorbate autoxidation and ascorbate-mediated .OH production, thereby indicating that these compounds suppress the activating effect of iron. Ascorbate autoxidation and ascorbate-dependent .OH formation, stimulated by Fe2+ (EDTA) were significantly inhibited by .OH scavengers, namely mannitol, thiourea and sodium formate, as well as by catalase and to a lesser extent by bovine serum albumin, superoxide dismutase (native and heat denatured) and heat denatured catalase.

Original languageEnglish
Pages (from-to)289-292
Number of pages4
JournalIndian Journal of Biochemistry and Biophysics
Issue number5
Publication statusPublished - 01-10-1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics


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