TY - JOUR
T1 - Bacterial synthesis of poly(hydroxybutyrate- co-hydroxyvalerate) using carbohydrate-rich mahua (Madhuca sp.) flowers
AU - Anil Kumar, P. K.
AU - Shamala, T. R.
AU - Kshama, L.
AU - Prakash, M. H.
AU - Joshi, G. J.
AU - Chandrashekar, A.
AU - Latha Kumari, K. S.
AU - Divyashree, M. S.
PY - 2007/7/1
Y1 - 2007/7/1
N2 - Aims: The objective of the present work was to utilize an unrefined natural substrate namely mahua (Madhuca sp.) flowers, as a carbon source for the production of bacterial polyhydroxyalkanoate (PHA) copolymer by Bacillus sp-256. Methods and Results: In the present work, three bacterial strains were tested for PHA production on mahua flower extract (to impart 20 g l-1 sugar) amongst which, Bacillus sp-256 produced higher concentration of PHA in its biomass (51%) compared with Rhizobium meliloti (31%) or Sphingomonas sp (22%). Biosynthesis of poly(hydroxybutyrate-co-hydroxyvalerate) - P(HB-co-HV) - of 90 : 10 mol% by Bacillus sp-256 was observed by gas chromatographic analysis of the polymer. Major component of the flower is sugars (57% on dry weight basis) and additionally it also contains proteins, vitamins, organic acids and essential oils. The bacterium utilized malic acid present in the substrate as a co-carbon source for the copolymer production. The flowers could be used in the form of aqueous extract or as whole flowers. PHA content of biomass (%) and yield (g l-1) in a 3·0-l stirred tank fermentor after 30 h of fermentation under constant pH (7) and dissolved oxygen content (40%) were 54% and 2.7 g l-1, respectively. Corresponding yields for control fermentation with sucrose as carbon source were 52% and 2.5 g l-1. The polymer was characterized by proton NMR. Conclusions: Utilization of mahua flowers, a natural substrate for bacterial fermentation aimed at PHA production, had additional advantage, as the sugars and organic acids present in the flowers were metabolized by Bacillus sp-256 to synthesize P(HB-co-HV) copolymer. Significance and Impact of the Study: Literature reports on utilization of suitable cheaper natural substrate for PHA copolymer production is scanty. Mahua flowers used in the present experiment is a cheaper carbon substrate compared with several commercial substrates and it is rich in main carbon as well as co-carbon sources that can be utilized by bacteria for PHA copolymer production.
AB - Aims: The objective of the present work was to utilize an unrefined natural substrate namely mahua (Madhuca sp.) flowers, as a carbon source for the production of bacterial polyhydroxyalkanoate (PHA) copolymer by Bacillus sp-256. Methods and Results: In the present work, three bacterial strains were tested for PHA production on mahua flower extract (to impart 20 g l-1 sugar) amongst which, Bacillus sp-256 produced higher concentration of PHA in its biomass (51%) compared with Rhizobium meliloti (31%) or Sphingomonas sp (22%). Biosynthesis of poly(hydroxybutyrate-co-hydroxyvalerate) - P(HB-co-HV) - of 90 : 10 mol% by Bacillus sp-256 was observed by gas chromatographic analysis of the polymer. Major component of the flower is sugars (57% on dry weight basis) and additionally it also contains proteins, vitamins, organic acids and essential oils. The bacterium utilized malic acid present in the substrate as a co-carbon source for the copolymer production. The flowers could be used in the form of aqueous extract or as whole flowers. PHA content of biomass (%) and yield (g l-1) in a 3·0-l stirred tank fermentor after 30 h of fermentation under constant pH (7) and dissolved oxygen content (40%) were 54% and 2.7 g l-1, respectively. Corresponding yields for control fermentation with sucrose as carbon source were 52% and 2.5 g l-1. The polymer was characterized by proton NMR. Conclusions: Utilization of mahua flowers, a natural substrate for bacterial fermentation aimed at PHA production, had additional advantage, as the sugars and organic acids present in the flowers were metabolized by Bacillus sp-256 to synthesize P(HB-co-HV) copolymer. Significance and Impact of the Study: Literature reports on utilization of suitable cheaper natural substrate for PHA copolymer production is scanty. Mahua flowers used in the present experiment is a cheaper carbon substrate compared with several commercial substrates and it is rich in main carbon as well as co-carbon sources that can be utilized by bacteria for PHA copolymer production.
UR - https://www.scopus.com/pages/publications/34250888639
UR - https://www.scopus.com/inward/citedby.url?scp=34250888639&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2672.2006.03221.x
DO - 10.1111/j.1365-2672.2006.03221.x
M3 - Article
C2 - 17584466
AN - SCOPUS:34250888639
SN - 1364-5072
VL - 103
SP - 204
EP - 209
JO - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
IS - 1
ER -