TY - JOUR
T1 - Basella alba stem extract integrated poly (vinyl alcohol)/chitosan composite films
T2 - A promising bio-material for wound healing
AU - D'souza, Oshin Jacintha
AU - Gasti, Tilak
AU - Hiremani, Vishram D.
AU - Pinto, Jennifer P.
AU - Contractor, Shafa S.
AU - Shettar, Arun K.
AU - Olivia, Diana
AU - Arakera, Suresh B.
AU - Masti, Saraswati P.
AU - Chougale, Ravindra B.
N1 - Funding Information:
The authors honestly express their gratitude to University Scientific & Instruments Centre (USIC), DST-SAIF and also DST-PURSE: PHASE-II (Grant No: SR/PURSE PHASE-2/13-G ) program, Karnatak University , Dharwad, India, for providing instrumentation services. The authors would like to acknowledge the Karnatak University , Dharwad, for providing financial assistance under university Seed Grant Programme for minor research project (No: KU/PMEB/2021-22/68 ). The authors also express their heartfelt gratitude to Dr. Saraswati P. Masti, Principal Investigator, DST-SERB Project No. SB/EMEQ-213/2014, Department of Chemistry, Karnatak Science College, Dharwad, Karnataka, India, for providing Universal Testing Machine (UTM) facility. One of the authors, Mrs. Oshin Jacintha D'souza would like to express her heartfelt thanks to Karnatak University, Dharwad, for providing university research studentship.
Funding Information:
The authors honestly express their gratitude to University Scientific & Instruments Centre (USIC), DST-SAIF and also DST-PURSE: PHASE-II (Grant No: SR/PURSE PHASE-2/13-G) program, Karnatak University, Dharwad, India, for providing instrumentation services. The authors would like to acknowledge the Karnatak University, Dharwad, for providing financial assistance under university Seed Grant Programme for minor research project (No: KU/PMEB/2021-22/68). The authors also express their heartfelt gratitude to Dr. Saraswati P. Masti, Principal Investigator, DST-SERB Project No. SB/EMEQ-213/2014, Department of Chemistry, Karnatak Science College, Dharwad, Karnataka, India, for providing Universal Testing Machine (UTM) facility. One of the authors, Mrs. Oshin Jacintha D'souza would like to express her heartfelt thanks to Karnatak University, Dharwad, for providing university research studentship.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2023/1/15
Y1 - 2023/1/15
N2 - Natural extract-based bio-composite material for wound healing is gaining much attention due to risk of infection and high cost of commercial wound dressing film causes serious problem on the human well-being. Herein, the study outlines the preparation of Poly (vinyl alcohol)/Chitosan/Basella alba stem extract (BAE) based bio-composite film through solvent casting technique and well characterized for wound healing application. Incorporation of BAE into Poly (vinyl alcohol)/Chitosan matrix has shown existence of secondary interactions confirmed by FT-IR analysis. Good morphology, thermal stability and significant improvement in flexibility (∼63.38 %) of the films were confirmed by SEM, TGA and Mechanical test results, respectively. Hydrophilic property (∼9.04 %), water vapor transmission rate (∼70.07 %), swelling ability (∼14.7 %) and degradation rate (∼14.04 %) were enhanced with increase in BAE content. In-vitro studies have shown good antibacterial activity against foremost infectious bacterial strains S. aureus and E. coli. Additionally, BAE integrated Poly (vinyl alcohol)/Chitosan film has amplified anti-inflammatory (∼79.38 %) property, hemocompatibility and excellent biocompatibility (94.9 %) was displayed by cytotoxicity results. Moreover, in-vitro scratch assay and cell adhesion test results illustrated prominent wound healing (96.5 %) and adhesion. Overall results of the present work proclaim that developed bio-composite film could be utilized as a biomaterial in wound care applications.
AB - Natural extract-based bio-composite material for wound healing is gaining much attention due to risk of infection and high cost of commercial wound dressing film causes serious problem on the human well-being. Herein, the study outlines the preparation of Poly (vinyl alcohol)/Chitosan/Basella alba stem extract (BAE) based bio-composite film through solvent casting technique and well characterized for wound healing application. Incorporation of BAE into Poly (vinyl alcohol)/Chitosan matrix has shown existence of secondary interactions confirmed by FT-IR analysis. Good morphology, thermal stability and significant improvement in flexibility (∼63.38 %) of the films were confirmed by SEM, TGA and Mechanical test results, respectively. Hydrophilic property (∼9.04 %), water vapor transmission rate (∼70.07 %), swelling ability (∼14.7 %) and degradation rate (∼14.04 %) were enhanced with increase in BAE content. In-vitro studies have shown good antibacterial activity against foremost infectious bacterial strains S. aureus and E. coli. Additionally, BAE integrated Poly (vinyl alcohol)/Chitosan film has amplified anti-inflammatory (∼79.38 %) property, hemocompatibility and excellent biocompatibility (94.9 %) was displayed by cytotoxicity results. Moreover, in-vitro scratch assay and cell adhesion test results illustrated prominent wound healing (96.5 %) and adhesion. Overall results of the present work proclaim that developed bio-composite film could be utilized as a biomaterial in wound care applications.
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U2 - 10.1016/j.ijbiomac.2022.11.130
DO - 10.1016/j.ijbiomac.2022.11.130
M3 - Article
C2 - 36403767
AN - SCOPUS:85142722341
SN - 0141-8130
VL - 225
SP - 673
EP - 686
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -