TY - JOUR
T1 - Bimodal UV photoacoustic and fluorescence sensor for breath analysis
AU - V.R., Nidheesh
AU - Mohapatra, Aswini Kumar
AU - Nayak, Rajesh
AU - V.K., Unnikrishnan
AU - Kartha, Vasudevan Baskaran
AU - Chidangil, Santhosh
N1 - Funding Information:
Mr. Nidheesh V.R is grateful to Manipal Academy of Higher Education (MAHE) for the Dr. TMA Pai Doctoral fellowship. The authors are thankful to the Manipal Academy of Higher Education for the financial support to procure components for assembling the PAS-LIF setup, DST-FIST Govt. of India and VGST GOVT. of Karnataka for other research facilities.
Publisher Copyright:
© 2023
PY - 2023/3/15
Y1 - 2023/3/15
N2 - Breath analysis using spectroscopy techniques is coming up as one of the non-invasive methods for disease diagnosis. Among several spectroscopic methods, photoacoustic spectroscopy (PAS) and laser-induced fluorescence (LIF) are used to obtain information on sample's absorption and emission characteristics, respectively. In the present work, we present breath analysis results using a bimodal sensor using 266 nm UV laser, which can be used for both PAS and LIF measurements of volatile organic compounds (VOCs). The system is optimized using standard VOC samples to show limits of detection of the order of ppb-s. Preliminary breath analysis has been carried out for breath samples from Asthma patients and healthy volunteers. Principal component analysis has shown that the two sets of PAS and LIF data of Asthma and normal breath samples are discriminated from each other as distinct clusters enabling clear discrimination. Match/no match study using M-distance and spectral residual gives a sensitivity of 90 % and specificity of 96 % for PAS, and 85 % sensitivity and 86 % specificity for LIF, using optimized M-distance cutoff values. The receiver operating curve (ROC) and the area under the curve (AUC)-ROC shows that the method is quite suitable for applying bimodal spectroscopy for breath analysis. A comparison of the breath study of the same volunteers has been carried out using an E-nose to confirm the validity of UV laser-based PAS-LIF bimodal sensor.
AB - Breath analysis using spectroscopy techniques is coming up as one of the non-invasive methods for disease diagnosis. Among several spectroscopic methods, photoacoustic spectroscopy (PAS) and laser-induced fluorescence (LIF) are used to obtain information on sample's absorption and emission characteristics, respectively. In the present work, we present breath analysis results using a bimodal sensor using 266 nm UV laser, which can be used for both PAS and LIF measurements of volatile organic compounds (VOCs). The system is optimized using standard VOC samples to show limits of detection of the order of ppb-s. Preliminary breath analysis has been carried out for breath samples from Asthma patients and healthy volunteers. Principal component analysis has shown that the two sets of PAS and LIF data of Asthma and normal breath samples are discriminated from each other as distinct clusters enabling clear discrimination. Match/no match study using M-distance and spectral residual gives a sensitivity of 90 % and specificity of 96 % for PAS, and 85 % sensitivity and 86 % specificity for LIF, using optimized M-distance cutoff values. The receiver operating curve (ROC) and the area under the curve (AUC)-ROC shows that the method is quite suitable for applying bimodal spectroscopy for breath analysis. A comparison of the breath study of the same volunteers has been carried out using an E-nose to confirm the validity of UV laser-based PAS-LIF bimodal sensor.
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U2 - 10.1016/j.snb.2022.133242
DO - 10.1016/j.snb.2022.133242
M3 - Article
AN - SCOPUS:85146010704
SN - 0925-4005
VL - 379
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
M1 - 133242
ER -