TY - JOUR
T1 - Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective
AU - Prabhakar, K.R.
AU - Veeresh, V.P.
AU - Vipan, K.
AU - Sudheer, M.
AU - Priyadarsini, K.I.
AU - Satish, R.B.S.S.
AU - Unnikrishnan, M.K.
N1 - Cited By :27
Export Date: 10 November 2017
CODEN: PYTOE
Correspondence Address: Unnikrishnan, M.K.; Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal, 576104, India; email: [email protected]
Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; hydroxyl radical, 3352-57-6; nitric oxide, 10102-43-9; thiocyanate potassium, 333-20-0; 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid, 28752-68-3; 2,2-diphenyl-1-picrylhydrazyl, 1898-66-4; Benzothiazoles; Free Radical Scavengers; Hydroxyl Radical, 3352-57-6; Nitric Oxide, 10102-43-9; Picrates; Plant Extracts; Sulfonic Acids
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PY - 2006
Y1 - 2006
N2 - The whole plant aqueous extract of Coronopus didymus Linn. was fractionated on the basis of polarity and resulting fractions were evaluated for free radical scavenging ability. The most non-polar fraction (CDF1) was found to be more active than other fractions in scavenging DPPH, ABTS{radical dot}-, nitric oxide and hydroxyl radicals in steady-state conditions. Stop-flow spectrometric studies showed 58.13% inhibition of 100 μM DPPH at a concentration of 150 μg/ml of CDF1 in 1000 s and 32.31% scavenging of 960 μM ABTS{radical dot}- at a concentration of 300 μg/ml of CDF1 in 100 s. The reaction of CDF1 with hydroxyl radicals produced by pulse radiolysis showed a transient spectrum with absorption peaks at 320, 390 and 400 nm, indicating the presence of flavonoids/related components. Competition kinetics with potassium thiocyanate against scavenging of hydroxyl radicals showed a reactivity of 0.1326 against thiocyanate. CDF1 also protected against Fenton reagent-induced calf thymus DNA damage at a concentration of 400 mg/ml indicating it to be the most potent fraction. © 2005 Elsevier GmbH. All rights reserved.
AB - The whole plant aqueous extract of Coronopus didymus Linn. was fractionated on the basis of polarity and resulting fractions were evaluated for free radical scavenging ability. The most non-polar fraction (CDF1) was found to be more active than other fractions in scavenging DPPH, ABTS{radical dot}-, nitric oxide and hydroxyl radicals in steady-state conditions. Stop-flow spectrometric studies showed 58.13% inhibition of 100 μM DPPH at a concentration of 150 μg/ml of CDF1 in 1000 s and 32.31% scavenging of 960 μM ABTS{radical dot}- at a concentration of 300 μg/ml of CDF1 in 100 s. The reaction of CDF1 with hydroxyl radicals produced by pulse radiolysis showed a transient spectrum with absorption peaks at 320, 390 and 400 nm, indicating the presence of flavonoids/related components. Competition kinetics with potassium thiocyanate against scavenging of hydroxyl radicals showed a reactivity of 0.1326 against thiocyanate. CDF1 also protected against Fenton reagent-induced calf thymus DNA damage at a concentration of 400 mg/ml indicating it to be the most potent fraction. © 2005 Elsevier GmbH. All rights reserved.
U2 - 10.1016/j.phymed.2005.07.003
DO - 10.1016/j.phymed.2005.07.003
M3 - Article
SN - 0944-7113
VL - 13
SP - 591
EP - 595
JO - Phytomedicine
JF - Phytomedicine
IS - 8
ER -