@article{1d3d2d84f97645739df0911f3ab7f09d,
title = "Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective",
abstract = "The whole plant aqueous extract of Coronopus didymus Linn. was fractionated on the basis of polarity and resulting fractions were evaluated for free radical scavenging ability. The most non-polar fraction (CDF1) was found to be more active than other fractions in scavenging DPPH, ABTS{radical dot}-, nitric oxide and hydroxyl radicals in steady-state conditions. Stop-flow spectrometric studies showed 58.13% inhibition of 100 μM DPPH at a concentration of 150 μg/ml of CDF1 in 1000 s and 32.31% scavenging of 960 μM ABTS{radical dot}- at a concentration of 300 μg/ml of CDF1 in 100 s. The reaction of CDF1 with hydroxyl radicals produced by pulse radiolysis showed a transient spectrum with absorption peaks at 320, 390 and 400 nm, indicating the presence of flavonoids/related components. Competition kinetics with potassium thiocyanate against scavenging of hydroxyl radicals showed a reactivity of 0.1326 against thiocyanate. CDF1 also protected against Fenton reagent-induced calf thymus DNA damage at a concentration of 400 mg/ml indicating it to be the most potent fraction. {\textcopyright} 2005 Elsevier GmbH. All rights reserved.",
author = "K.R. Prabhakar and V.P. Veeresh and K. Vipan and M. Sudheer and K.I. Priyadarsini and R.B.S.S. Satish and M.K. Unnikrishnan",
note = "Cited By :27 Export Date: 10 November 2017 CODEN: PYTOE Correspondence Address: Unnikrishnan, M.K.; Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal, 576104, India; email: mkunnikrishnan@gmail.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; hydroxyl radical, 3352-57-6; nitric oxide, 10102-43-9; thiocyanate potassium, 333-20-0; 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid, 28752-68-3; 2,2-diphenyl-1-picrylhydrazyl, 1898-66-4; Benzothiazoles; Free Radical Scavengers; Hydroxyl Radical, 3352-57-6; Nitric Oxide, 10102-43-9; Picrates; Plant Extracts; Sulfonic Acids References: Adelman, R., Saul, R.L., Ames, B., Oxidative damage to DNA: relation to species metabolic rate and life-span (1988) Proc. Natl. Acad.Sci. USA, 85, pp. 2706-2708; Clardy, J., Walsh, C., Lessons from natural molecules (review) (2004) Nature, 432, pp. 829-837; De Ruiz, R.E.L., Fusco, M., Sosa A and Ruiz, S.O., Constituents of Coronopus didymus (1994) Fitoterpia, 65, pp. 181-182; Inam ul, H., Composition of essential oil of Coronopus didymus seeds (1989) J. Chem. Soc. Pakistan, 11, pp. 80-81; Kunchandy, E., Rao, M.N.A., Effect of curcumin on hydroxyl radical generation through Fenton reaction (1989) Int. J. Pharmaceut., 57, pp. 173-176; Lu, C., Koropatnick, J., George, C.M., Roles of vitamin C in radiation-induced DNA damage in presence and absence of copper (2001) Chemico-Biol. Interact., 137, pp. 75-88; McDowall, F.H., Mortan, I.D., McDowell, A.K.R., Land-cress taint in cream and butter (1947) NZ J. Sci. Technol. A, 28, pp. 305-307; Mishra, B., Priyadarsini, K.I., Sudheer Kumar, M., Unnikrishnan, M.K., Effect of O-glycosilation on the antioxidant activity and free radical reactions of a plant flavonoid, Chrysoeriol (2003) Bioorg. Med. Chem., 11 (13), pp. 2677-2685; Prabhakar, K.R., Srinivasan, K.K., Padma, G.M.R., Chemical investigation, anti-inflammatory and wound healing properties of Coronopus didymus (2002) Pharm. Biol., 40, pp. 490-493; Prieto, P., Pineda, M., Aguilar, M., Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E (1999) Anal. Biochem., 269, pp. 337-341; Pryor, W.A., Porter, N.A., Suggested mechanisms for the production of 4-hydroxy-2-nonenal from the autoxidation of polyunsaturated fatty acids (1990) Free Radic. Biol. Med., 8, pp. 541-543; Raj Sekhar Narla, Rao, M.N.A., Scavenging of free radicals and inhibition of lipid peroxidation by 3-phenyl syndone (1995) J. Pharm. Pharmacol., 47, pp. 623-625; Spinks, J.W.T., Woods, R.J., (1990) An Introduction to Radiation Chemistry, , Wiley, New York; Sudheer Kumar, M., Jagadish, S.R., Kiran Babu, S., Unnikrishnan, M.K., In-vitro evaluation of antioxidant properties of Cocus nucifera Linn Water (2003) Nahrung/Food, 47 (2), pp. 126-131; Uma Devi, P., Ganasoundari, A., Vrinda, B., Srinivasan, K.K., Unnikrishnan, M.K., Radiation protection by the Ocimum flavonoids Orientin and Vicenin: mechanism of action (2000) Radiat. Res., 154, pp. 455-460; Weiss, J.F., Landauer, M.R., Protection against ionizing radiation by antioxidant nutrients and Phytochemicals (2003) Toxicology, 189, pp. 1-20",
year = "2006",
doi = "10.1016/j.phymed.2005.07.003",
language = "English",
volume = "13",
pages = "591--595",
journal = "Phytomedicine",
issn = "0944-7113",
publisher = "Urban und Fischer Verlag Jena",
number = "8",
}