Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective

K.R. Prabhakar, V.P. Veeresh, K. Vipan, M. Sudheer, K.I. Priyadarsini, R.B.S.S. Satish, M.K. Unnikrishnan

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41 Citations (Scopus)


The whole plant aqueous extract of Coronopus didymus Linn. was fractionated on the basis of polarity and resulting fractions were evaluated for free radical scavenging ability. The most non-polar fraction (CDF1) was found to be more active than other fractions in scavenging DPPH, ABTS{radical dot}-, nitric oxide and hydroxyl radicals in steady-state conditions. Stop-flow spectrometric studies showed 58.13% inhibition of 100 μM DPPH at a concentration of 150 μg/ml of CDF1 in 1000 s and 32.31% scavenging of 960 μM ABTS{radical dot}- at a concentration of 300 μg/ml of CDF1 in 100 s. The reaction of CDF1 with hydroxyl radicals produced by pulse radiolysis showed a transient spectrum with absorption peaks at 320, 390 and 400 nm, indicating the presence of flavonoids/related components. Competition kinetics with potassium thiocyanate against scavenging of hydroxyl radicals showed a reactivity of 0.1326 against thiocyanate. CDF1 also protected against Fenton reagent-induced calf thymus DNA damage at a concentration of 400 mg/ml indicating it to be the most potent fraction. © 2005 Elsevier GmbH. All rights reserved.
Original languageEnglish
Pages (from-to)591-595
Number of pages5
Issue number8
Publication statusPublished - 2006


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