The application of non-biodegradable plastics has raised severe environmental concerns over the past few decades. The medium chain length polyhydroxyalkanoates (mcl-PHA) are biodegradable elastomeric polymers produced by the bacteria Pseudomonas, that can potentially solve this crisis. The industrial production of mcl-PHA is limited by low PHA yield of wild bacterial strains and the increased time the bacteria takes to achieve maximum PHA yield. Hence the present study focuses on the early PHA production in Pseudomonas aeruginosa MCC 5300. Pseudomonas aeruginosa MCC 5300 can grow to a high biomass and produce PHA within a short duration of 16 h of growth, reaching up to 75.6 % cell dry weight in media containing only oleic acid as a carbon source. A high amount of PHA production in such a short time was not reported in any wild-type bacteria cultured in nutrient-defined media. The mcl-PHA was a copolymer of 3-hydroxydecanoate and 3-hydroxydodecanoate.
All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal