TY - JOUR
T1 - Bisphenol A exposure modulates reproductive and endocrine system, mitochondrial function and cellular senescence in female adult rats
T2 - A hallmarks of polycystic ovarian syndrome phenotype
AU - Prabhu, Navya B.
AU - Adiga, Divya
AU - Kabekkodu, Shama Prasada
AU - Bhat, Shashikala K.
AU - Satyamoorthy, Kapaettu
AU - Rai, Padmalatha S.
N1 - Funding Information:
This work was supported by the Manipal Academy of Higher Education, Manipal and Indian Council of Medical Research ( ICMR ) ( 2020-6122/CMB-BMS ).
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/11
Y1 - 2022/11
N2 - Bisphenol A (BPA) mimics estrogen and consequently suspected to be detrimental to female reproductive system. Biomonitoring confirms the BPA burden in body leading to a complex condition called polycystic ovarian syndrome (PCOS) which is frequently attributed to female infertility. Due to unclear precise molecular pathomechanisms of BPA in PCOS, we intend to examine the molecular mechanisms of the reproductive, endocrine, mitochondrial features, and cellular senescence in BPA-treated rats. We analyzed vaginal smears and ovarian follicles using microscope, assessed sex hormones by ELISA, analyzed BPA target gene expression by semi-quantitative RT-PCR, assessed senescence induction by β-galactosidase staining and immunofluorescence in BPA-treated rats. Our data showed hormonal imbalance, impaired folliculogenesis, abnormal expression patterns of target genes, CDKN2A overexpression and enhanced ROS levels in BPA-treated rats. This study provides insights on the effects of BPA exposure on ovulatory, hormonal, mitochondrial dysfunction, and senescence that benefit in better understanding of PCOS induced by BPA.
AB - Bisphenol A (BPA) mimics estrogen and consequently suspected to be detrimental to female reproductive system. Biomonitoring confirms the BPA burden in body leading to a complex condition called polycystic ovarian syndrome (PCOS) which is frequently attributed to female infertility. Due to unclear precise molecular pathomechanisms of BPA in PCOS, we intend to examine the molecular mechanisms of the reproductive, endocrine, mitochondrial features, and cellular senescence in BPA-treated rats. We analyzed vaginal smears and ovarian follicles using microscope, assessed sex hormones by ELISA, analyzed BPA target gene expression by semi-quantitative RT-PCR, assessed senescence induction by β-galactosidase staining and immunofluorescence in BPA-treated rats. Our data showed hormonal imbalance, impaired folliculogenesis, abnormal expression patterns of target genes, CDKN2A overexpression and enhanced ROS levels in BPA-treated rats. This study provides insights on the effects of BPA exposure on ovulatory, hormonal, mitochondrial dysfunction, and senescence that benefit in better understanding of PCOS induced by BPA.
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U2 - 10.1016/j.etap.2022.104010
DO - 10.1016/j.etap.2022.104010
M3 - Article
AN - SCOPUS:85141338307
SN - 1382-6689
VL - 96
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
M1 - 104010
ER -