TY - JOUR
T1 - Blue LED light exposure induces metabolic rewiring in vitreous tissues in rat models
AU - Nagarajan, T. N.
AU - Joshi, Manjunath B.
AU - Ballae Ganeshrao, Shonraj
AU - Valiathan, Manna
AU - Surendran, Sudarshan
N1 - Funding Information:
This study is funded by science & engineering research board (SERB) (a statutory body of the Department of Science & Technology, Government of India) EMR/2017/004341 .
Publisher Copyright:
© 2022 The Authors
PY - 2022/6
Y1 - 2022/6
N2 - Blue light exposure induced retinal damage has been extensively studied. Retinal damages are closely associated with cellular and biochemical changes occurring in the vitreous and hence, understanding metabolic changes in vitreous tissue might serve as clinical importance to indicate ocular/vision health. In the present study, we have investigated the influence of blue light-emitting diodes (LED) on the vitreous metabolome and further, we show amelioration of altered metabolite levels upon blue light blocking lenses (BBLs). A total of n = 24 (n = 6 in each group; control, light exposure without lenses, two different BBLs) male Wistar rats were subjected to blue light exposure (LEDs, 450–500 lx) without or with BBLs (400–490 nm) for 28 days on a 12:12 h light–dark cycle. Post-exposure the vitreous fluid aspirated was subjected for untargeted liquid chromatography/mass spectrometry-based metabolomics analysis. Analyzed vitreous revealed blue light significantly modulated metabolites such as Tyrosine, L-Histidine (p = 0.02), L-Isoleucine (p = 0.007), Valine (p = 0.04) and D-Proline (P < 0.001) along with affected homocysteine degradation and nitric oxide signalling pathway. Our findings suggest exposure to blue LED poses a significant hazard to the vitreous as it alters the vitreous metabolites, and it is partially ameliorated by commercially available BBLs.
AB - Blue light exposure induced retinal damage has been extensively studied. Retinal damages are closely associated with cellular and biochemical changes occurring in the vitreous and hence, understanding metabolic changes in vitreous tissue might serve as clinical importance to indicate ocular/vision health. In the present study, we have investigated the influence of blue light-emitting diodes (LED) on the vitreous metabolome and further, we show amelioration of altered metabolite levels upon blue light blocking lenses (BBLs). A total of n = 24 (n = 6 in each group; control, light exposure without lenses, two different BBLs) male Wistar rats were subjected to blue light exposure (LEDs, 450–500 lx) without or with BBLs (400–490 nm) for 28 days on a 12:12 h light–dark cycle. Post-exposure the vitreous fluid aspirated was subjected for untargeted liquid chromatography/mass spectrometry-based metabolomics analysis. Analyzed vitreous revealed blue light significantly modulated metabolites such as Tyrosine, L-Histidine (p = 0.02), L-Isoleucine (p = 0.007), Valine (p = 0.04) and D-Proline (P < 0.001) along with affected homocysteine degradation and nitric oxide signalling pathway. Our findings suggest exposure to blue LED poses a significant hazard to the vitreous as it alters the vitreous metabolites, and it is partially ameliorated by commercially available BBLs.
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U2 - 10.1016/j.jksus.2022.101986
DO - 10.1016/j.jksus.2022.101986
M3 - Article
AN - SCOPUS:85127330776
SN - 1018-3647
VL - 34
JO - Journal of King Saud University - Science
JF - Journal of King Saud University - Science
IS - 4
M1 - 101986
ER -