C-MEMS Derived Glassy Carbon Glucose Sensor

Diabetes is one of the most severe health diseases in the world. More than 150 million people worldwide suffer from diabetes caused by an abnormal glucose concentration in their blood and tissues. The analysis of glucose levels in the body is significant, primarily due to diabetes mellitus. Therefore, accurately detecting blood glucose is crucial for diagnosing, treating, and managing diabetes. Diabetic patients can self-manage their condition by monitoring their glucose levels. The electrochemical glucose biosensor has several advantages, including recognizing glucose specifically, low price, ease of sensor manufacture, correctness, portability, and easy operation. We have developed glassy carbon electrodes (GCE) with conventional carbon-micro-electromechanical (C-MEMS) procedures for glucose sensing. SU-8 photoresist was used as the carbon precursor. The fabricated C-MEMS-derived GCE surface has been functionalized with chitosan and glucose oxidase. Glucose oxidase is a well-known enzyme for oxidizing glucose into gluconic acid and H2O2. This reaction kinetics has been recorded with increasing glucose concentration using electrochemical analysis. In summary, we have presented an electrochemical glucose biosensor in a single-step immobilization protocol of glucose oxidase on the glassy carbon electrode (GCE) surface and obtained satisfactory responses for 1 mM to 10 mM glucose solutions.