Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells

Raghavendra Upadhya; Leelavathi N. Madhu; Sahithi Attaluri; Daniel Gitai; Marisa R. Pinson; Maheedhar Kodali; Geetha A. Shetty; Gabriele Zanirati; Smrithi Kumar; Bing Shuai; Susan T. Weintraub; Ashok K. Shetty

Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells

Raghavendra Upadhya
, Leelavathi N. Madhu
, Sahithi Attaluri
, Daniel Gitai
, Marisa R. Pinson
, Maheedhar Kodali
, Geetha A. Shetty
, Gabriele Zanirati
, Smrithi Kumar
, Bing Shuai
, Susan T. Weintraub
, Ashok K. Shetty

Department of Biotherapeutics Research (DBR)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Neural stem cell (NSC) therapy has shown promise for brain repair after injury or disease mostly through bystander effects. Nevertheless, the translation of NSCs derived from human induced pluripotent stem cells (hiPSCs) to the clinic remain constrained due to safety issues, which include immunogenic risks, tumorigenesis potential, and incomplete differentiation. A way to avoid these issues is by using extracellular vesicles (EVs) generated from NSCs, as NSC-EVs likely have similar neuroprotective properties as NSCs and are amenable for non-invasive administration as an autologous or allogeneic off-the-shelf product. However, this would require reliable purification and characterization processes, and testing of EVs for composition and biological properties.

Original language	English
Title of host publication	Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells
Place of Publication	Philadalphia, USA.
Publisher	International Society for Extracellular Vesicles
Publication status	Published - 2020

Publication series

Name	ANNUAL MEETING ISEV 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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Upadhya, R., Madhu, L. N., Attaluri, S., Gitai, D., Pinson, M. R., Kodali, M., Shetty, G. A., Zanirati, G., Kumar, S., Shuai, B., Weintraub, S. T., & Shetty, A. K. (2020). Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells. In Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells (ANNUAL MEETING ISEV 2020). International Society for Extracellular Vesicles. https://www.eventscribe.com/2020/ISEV/fsPopup.asp?efp=TUFSSEtGRkw5NzM3&PosterID=279812&rnd=0.8992549&mode=posterinfo

Upadhya, Raghavendra ; Madhu, Leelavathi N. ; Attaluri, Sahithi et al. / Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells. Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells. Philadalphia, USA. : International Society for Extracellular Vesicles, 2020. (ANNUAL MEETING ISEV 2020).

@inproceedings{e8eb5a4877d1476889e24d14a34e30f5,

title = "Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells",

abstract = "Neural stem cell (NSC) therapy has shown promise for brain repair after injury or disease mostly through bystander effects. Nevertheless, the translation of NSCs derived from human induced pluripotent stem cells (hiPSCs) to the clinic remain constrained due to safety issues, which include immunogenic risks, tumorigenesis potential, and incomplete differentiation. A way to avoid these issues is by using extracellular vesicles (EVs) generated from NSCs, as NSC-EVs likely have similar neuroprotective properties as NSCs and are amenable for non-invasive administration as an autologous or allogeneic off-the-shelf product. However, this would require reliable purification and characterization processes, and testing of EVs for composition and biological properties.",

author = "Raghavendra Upadhya and Madhu, \{Leelavathi N.\} and Sahithi Attaluri and Daniel Gitai and Pinson, \{Marisa R.\} and Maheedhar Kodali and Shetty, \{Geetha A.\} and Gabriele Zanirati and Smrithi Kumar and Bing Shuai and Weintraub, \{Susan T.\} and Shetty, \{Ashok K.\}",

year = "2020",

language = "English",

series = "ANNUAL MEETING ISEV 2020",

publisher = "International Society for Extracellular Vesicles",

booktitle = "Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells",

}

Upadhya, R, Madhu, LN, Attaluri, S, Gitai, D, Pinson, MR, Kodali, M, Shetty, GA, Zanirati, G, Kumar, S, Shuai, B, Weintraub, ST & Shetty, AK 2020, Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells. in Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells. ANNUAL MEETING ISEV 2020, International Society for Extracellular Vesicles, Philadalphia, USA. <https://www.eventscribe.com/2020/ISEV/fsPopup.asp?efp=TUFSSEtGRkw5NzM3&PosterID=279812&rnd=0.8992549&mode=posterinfo>

Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells. / Upadhya, Raghavendra; Madhu, Leelavathi N.; Attaluri, Sahithi et al.
Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells. Philadalphia, USA.: International Society for Extracellular Vesicles, 2020. (ANNUAL MEETING ISEV 2020).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells

AU - Upadhya, Raghavendra

AU - Madhu, Leelavathi N.

AU - Attaluri, Sahithi

AU - Gitai, Daniel

AU - Pinson, Marisa R.

AU - Kodali, Maheedhar

AU - Shetty, Geetha A.

AU - Zanirati, Gabriele

AU - Kumar, Smrithi

AU - Shuai, Bing

AU - Weintraub, Susan T.

AU - Shetty, Ashok K.

PY - 2020

Y1 - 2020

N2 - Neural stem cell (NSC) therapy has shown promise for brain repair after injury or disease mostly through bystander effects. Nevertheless, the translation of NSCs derived from human induced pluripotent stem cells (hiPSCs) to the clinic remain constrained due to safety issues, which include immunogenic risks, tumorigenesis potential, and incomplete differentiation. A way to avoid these issues is by using extracellular vesicles (EVs) generated from NSCs, as NSC-EVs likely have similar neuroprotective properties as NSCs and are amenable for non-invasive administration as an autologous or allogeneic off-the-shelf product. However, this would require reliable purification and characterization processes, and testing of EVs for composition and biological properties.

AB - Neural stem cell (NSC) therapy has shown promise for brain repair after injury or disease mostly through bystander effects. Nevertheless, the translation of NSCs derived from human induced pluripotent stem cells (hiPSCs) to the clinic remain constrained due to safety issues, which include immunogenic risks, tumorigenesis potential, and incomplete differentiation. A way to avoid these issues is by using extracellular vesicles (EVs) generated from NSCs, as NSC-EVs likely have similar neuroprotective properties as NSCs and are amenable for non-invasive administration as an autologous or allogeneic off-the-shelf product. However, this would require reliable purification and characterization processes, and testing of EVs for composition and biological properties.

M3 - Conference contribution

T3 - ANNUAL MEETING ISEV 2020

BT - Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells

PB - International Society for Extracellular Vesicles

CY - Philadalphia, USA.

ER -

Upadhya R, Madhu LN, Attaluri S, Gitai D, Pinson MR, Kodali M et al. Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells. In Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells. Philadalphia, USA.: International Society for Extracellular Vesicles. 2020. (ANNUAL MEETING ISEV 2020).

Characterization, composition, and biological properties of extracellular vesicles generated from human induced pluripotent stem cell-derived neural stem cells

Abstract

Publication series

UN SDGs

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