TY - JOUR
T1 - Characterization of biosurfactant produced in response to petroleum crude oil stress by Bacillus sp. WD22 in marine environment
AU - Goveas, Louella Concepta
AU - Selvaraj, Raja
AU - Sajankila, Shyama Prasad
N1 - Funding Information:
This work is funded by the Vision Group of Science and Technology, Govt. of Karnataka (VGST-GoK) RGS-F grant (GRD No 846/315).
Funding Information:
LCG thanks the Vision Group of Science and Technology, Govt. of Karnataka (VGST-GoK) for funding this research work under RGS-F grant (GRD No 846/315).
Publisher Copyright:
© 2022, The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.
PY - 2022/12
Y1 - 2022/12
N2 - Bacillus sp. WD22, previously isolated from refinery effluent, degraded 71% of C8 hydrocarbons present in 1.0% v/v PCO in seawater (control medium), which reduced to 16.3%, on addition of yeast extract. The bacteria produced a biosurfactant in both media, whose surface was observed to be amorphous in nature under FESEM-EDAX analysis. The biosurfactant was characterized as a linear surfactin by LCMS and FT-IR analysis. The critical micelle concentration was observed as 50 mg/L and 60 mg/L at which the surface tension of water was reduced to 30 mN/m. Purified biosurfactant could emulsify petroleum-based oils and vegetable oils effectively and was stable at all tested conditions of pH, salinity and temperature up to 80 °C. The biosurfactant production was found to be mixed growth associated in control medium, while it was strictly growth associated in medium with yeast extract as studied by the Leudeking-Piret model.
AB - Bacillus sp. WD22, previously isolated from refinery effluent, degraded 71% of C8 hydrocarbons present in 1.0% v/v PCO in seawater (control medium), which reduced to 16.3%, on addition of yeast extract. The bacteria produced a biosurfactant in both media, whose surface was observed to be amorphous in nature under FESEM-EDAX analysis. The biosurfactant was characterized as a linear surfactin by LCMS and FT-IR analysis. The critical micelle concentration was observed as 50 mg/L and 60 mg/L at which the surface tension of water was reduced to 30 mN/m. Purified biosurfactant could emulsify petroleum-based oils and vegetable oils effectively and was stable at all tested conditions of pH, salinity and temperature up to 80 °C. The biosurfactant production was found to be mixed growth associated in control medium, while it was strictly growth associated in medium with yeast extract as studied by the Leudeking-Piret model.
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U2 - 10.1007/s42770-022-00811-4
DO - 10.1007/s42770-022-00811-4
M3 - Article
AN - SCOPUS:85137436764
SN - 1517-8382
VL - 53
SP - 2015
EP - 2025
JO - Revista de Microbiologia
JF - Revista de Microbiologia
IS - 4
ER -
