CHEMICAL FINGERPRINTING OF PIPERINE AND TANNIC ACID IN AN AYURVEDIC FORMULATION OF DASANAKANTHI CHURNAM USING RP-HPLC

Dental diseases are one of the significant public health problems globally. Herbal medicines for managing oral diseases are considered an effective alternative to synthetic compounds due to their lower side effect. Acacia catechu, Piper nigrum, Piper longum, and Piper cubeba are vital components of Dasanakanthi Churnam used to control and prevent oral inflammations in dentistry. Tannic acid and Piperine are major markers for the chemical fingerprinting of Dasanakanthi Churnam. They serve as quality indicating parameters for Dasanakanthi Churnam because of their pharmacological action. Development and validation of a stability-indicating assay method using RP-HPLC become an integral part of simultaneous estimation of Tannic acid and Piperine in Dasanakanthi Churnam. Stability indicating the method is established by exposing markers to various stress conditions such as alkaline hydrolysis, photolytic, oxidative and thermal conditions. Stress testing of the markers can help identify the likely degradation products and validate the stability indicating the power of the analytical procedures used. Tannic acid and Piperine were effectively separated at 5.2 and 22.5 minutes respectively using a PDA detector without interference from any degradant and impurities. This research gives a scope to further carry out the stability studies such as accelerated, intermediate, and long-term studies for Dasanakanthi Churnam.