TY - JOUR
T1 - Chitosan functionalized halloysite nanotube/poly (caprolactone) nanocomposites for wound healing application
AU - Kouser, Sabia
AU - Prabhu, Ashwini
AU - Sheik, Sareen
AU - Prashantha, Kalappa
AU - Nagaraja, G. K.
AU - Neetha D'souza, Josline
AU - Navada, K. M.
AU - Manasa, D. J.
N1 - Funding Information:
The authors would like to thank DST-PURSE Mangalore University for the instrument facility and the Karnataka Government VGST project for supporting our research. Also thanks to the Directorate of Minorities, Karnataka Government for the award of the fellowship. For proving instrument facility (mechanical testing) the authors express their gratitude to Dr. Saraswasti P. Masti, Investigator Principal (DST-SERB, Project No.SB/EMEQ-213/2014), Chemistry department, Karnataka Science College, Dharwad-580001, Karnataka.
Publisher Copyright:
© 2021 The Author(s)
PY - 2021/12/1
Y1 - 2021/12/1
N2 - The present study depicts the surface functionalization of halloysite nanotubes (HNTs) with chitosan and intruded in the poly(caprolactone) (PCL) matrix to synthesize nanocomposites for the biomedical field. The solution casting method was used to fabricate the nanocomposite films, and investigated by various characterization techniques. The films exhibited enhanced thermal and mechanical properties. FE-SEM and AFM analyses confirmed the uniform dispersion of the nanofiller and increased roughness of the films. The nanocomposite films were also examined for swelling ratio, in-vitro enzymatic degradation, and anti-inflammatory activity. The MTT assay performed using NIH3T3 cell lines for the nanocomposite films has revealed significant cell proliferation (127±1.21 for 5wt%). Additionally, the cell adhesion assay also reflected the cytocompatibility nature of the films. The scratch assay was performed to see the capability of the films for wound closure observed that the 5wt% film showed a greater rate of fibroblast cell migration (31.49±0.89) compared to pristine PCL film. The hemocompatibility of these films were determined through HRBCMS assay. The biological activity results revealed that the nanocomposites exhibited delayed enzymatic degradability and hemocompatibility, besides improved cell proliferation, cell adhesion, and cell migration efficacy with respect to NIH3T3 cells. Therefore, the fabricated nanocomposite films can be potentially used in wound healing applications after appropriate clinical validations to test their efficacy.
AB - The present study depicts the surface functionalization of halloysite nanotubes (HNTs) with chitosan and intruded in the poly(caprolactone) (PCL) matrix to synthesize nanocomposites for the biomedical field. The solution casting method was used to fabricate the nanocomposite films, and investigated by various characterization techniques. The films exhibited enhanced thermal and mechanical properties. FE-SEM and AFM analyses confirmed the uniform dispersion of the nanofiller and increased roughness of the films. The nanocomposite films were also examined for swelling ratio, in-vitro enzymatic degradation, and anti-inflammatory activity. The MTT assay performed using NIH3T3 cell lines for the nanocomposite films has revealed significant cell proliferation (127±1.21 for 5wt%). Additionally, the cell adhesion assay also reflected the cytocompatibility nature of the films. The scratch assay was performed to see the capability of the films for wound closure observed that the 5wt% film showed a greater rate of fibroblast cell migration (31.49±0.89) compared to pristine PCL film. The hemocompatibility of these films were determined through HRBCMS assay. The biological activity results revealed that the nanocomposites exhibited delayed enzymatic degradability and hemocompatibility, besides improved cell proliferation, cell adhesion, and cell migration efficacy with respect to NIH3T3 cells. Therefore, the fabricated nanocomposite films can be potentially used in wound healing applications after appropriate clinical validations to test their efficacy.
UR - https://www.scopus.com/pages/publications/85116014619
UR - https://www.scopus.com/inward/citedby.url?scp=85116014619&partnerID=8YFLogxK
U2 - 10.1016/j.apsadv.2021.100158
DO - 10.1016/j.apsadv.2021.100158
M3 - Article
AN - SCOPUS:85116014619
SN - 2666-5239
VL - 6
JO - Applied Surface Science Advances
JF - Applied Surface Science Advances
M1 - 100158
ER -