Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec

Mahadev Rao; Bradley A. Carlson; Sergey V. Novoselov; Donald P. Weeks; Vadim N. Gladyshev; Dolph L. Hatfield

doi:10.1261/rna.5510503

Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec

Mahadev Rao, Bradley A. Carlson, Sergey V. Novoselov, Donald P. Weeks, Vadim N. Gladyshev, Dolph L. Hatfield

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found in Chlamydomonas reinhardtii, a member of the plant kingdom. Herein, we used RT-PCR to determine the sequence of C. reinhardtii Sec tRNA^[Ser]Sec, the first non-animal eukaryotic Sec tRNA^[Ser]Sec sequence. Like its animal counterpart, it is 90 nucleotides in length, is aminoacylated with serine by seryl-tRNA synthetase, and decodes specifically UGA. Evolutionary analyses of known Sec tRNAs identify the C. reinhardtii form as the most diverged eukaryotic Sec TRNA^[Ser]Sec and reveal a common origin for this tRNA in bacteria, archaea, and eukaryotes.

Original language	English
Pages (from-to)	923-930
Number of pages	8
Journal	RNA
Volume	9
Issue number	8
DOIs	https://doi.org/10.1261/rna.5510503
Publication status	Published - 01-08-2003

All Science Journal Classification (ASJC) codes

Genetics
Molecular Biology

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title = "Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec",

abstract = "Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found in Chlamydomonas reinhardtii, a member of the plant kingdom. Herein, we used RT-PCR to determine the sequence of C. reinhardtii Sec tRNA[Ser]Sec, the first non-animal eukaryotic Sec tRNA[Ser]Sec sequence. Like its animal counterpart, it is 90 nucleotides in length, is aminoacylated with serine by seryl-tRNA synthetase, and decodes specifically UGA. Evolutionary analyses of known Sec tRNAs identify the C. reinhardtii form as the most diverged eukaryotic Sec TRNA[Ser]Sec and reveal a common origin for this tRNA in bacteria, archaea, and eukaryotes.",

author = "Mahadev Rao and Carlson, {Bradley A.} and Novoselov, {Sergey V.} and Weeks, {Donald P.} and Gladyshev, {Vadim N.} and Hatfield, {Dolph L.}",

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T1 - Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec

AU - Rao, Mahadev

AU - Carlson, Bradley A.

AU - Novoselov, Sergey V.

AU - Weeks, Donald P.

AU - Gladyshev, Vadim N.

AU - Hatfield, Dolph L.

PY - 2003/8/1

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AB - Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found in Chlamydomonas reinhardtii, a member of the plant kingdom. Herein, we used RT-PCR to determine the sequence of C. reinhardtii Sec tRNA[Ser]Sec, the first non-animal eukaryotic Sec tRNA[Ser]Sec sequence. Like its animal counterpart, it is 90 nucleotides in length, is aminoacylated with serine by seryl-tRNA synthetase, and decodes specifically UGA. Evolutionary analyses of known Sec tRNAs identify the C. reinhardtii form as the most diverged eukaryotic Sec TRNA[Ser]Sec and reveal a common origin for this tRNA in bacteria, archaea, and eukaryotes.

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Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec

Abstract

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