TY - JOUR
T1 - Chromatin-bound PCNA as S-phase marker in mononuclear blood cells of patients with acute lymphoblastic leukaemia or multiple myeloma
AU - Zölzer, F.
AU - Basu, O.
AU - Devi, P. U.
AU - Mohanty, S. P.
AU - Streffer, C.
PY - 2010/12/1
Y1 - 2010/12/1
N2 - Objectives: Proliferating cell nuclear antigen (PCNA) has often been used as a marker to aid assessment of tumour growth fraction. This paper addresses the question of whether it can be used as an S-phase marker, when the non-chromatin-bound form of the protein is removed by pepsin treatment.Materials and methods: Cytofluorometric measurements were carried out after immunofluorescence staining of PCNA and counterstaining of DNA. S-phase fraction was determined with the help of windows on PCNA versus DNA scattergrams, or mathematically from DNA histograms.Results: S-phase fractions obtained using the two methods correlated well, but did not always agree, exact discrepancies depending on the mathematical model used for histogram analysis.Conclusions: Determination of S-phase fractions with the help of PCNA immunofluorescence staining is possible, and probably more reliable than calculation of S-fractions from DNA histograms. It thus offers an alternative to assays involving BrdU labelling in vivo.
AB - Objectives: Proliferating cell nuclear antigen (PCNA) has often been used as a marker to aid assessment of tumour growth fraction. This paper addresses the question of whether it can be used as an S-phase marker, when the non-chromatin-bound form of the protein is removed by pepsin treatment.Materials and methods: Cytofluorometric measurements were carried out after immunofluorescence staining of PCNA and counterstaining of DNA. S-phase fraction was determined with the help of windows on PCNA versus DNA scattergrams, or mathematically from DNA histograms.Results: S-phase fractions obtained using the two methods correlated well, but did not always agree, exact discrepancies depending on the mathematical model used for histogram analysis.Conclusions: Determination of S-phase fractions with the help of PCNA immunofluorescence staining is possible, and probably more reliable than calculation of S-fractions from DNA histograms. It thus offers an alternative to assays involving BrdU labelling in vivo.
UR - http://www.scopus.com/inward/record.url?scp=78049480266&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78049480266&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2184.2010.00707.x
DO - 10.1111/j.1365-2184.2010.00707.x
M3 - Article
C2 - 21039996
AN - SCOPUS:78049480266
SN - 0960-7722
VL - 43
SP - 579
EP - 583
JO - Cell and Tissue Kinetics
JF - Cell and Tissue Kinetics
IS - 6
ER -