Comparative evaluation of the efficacy of “advanced platelet‑rich fibrin plus” and enamel matrix derivative on proliferation and migration of periodontal ligament fibroblasts – An in vitro study

Background: Platelet concentrates and enamel matrix derivatives (EMD) have been used extensively for periodontal regenerative procedures. Modifications in processing protocols have resulted in a variety of concentrated platelet formulations. The present study evaluated the combination of a modified platelet concentrate, advanced platelet-rich fibrin plus (A-PRF+), with Emdogain^® on the proliferation and migration of human periodontal ligament fibroblasts. Materials and Methods: The study utilized an in vitro scratch and MTT assay for the stated purpose. We prepared A-PRF+ from healthy volunteer blood samples and procured Emdogain^® from Straumann India Pvt. Ltd. We compared A-PRF+, EMD, and the combination with distilled water for cell migration, proliferation, and wound closure at 0, 24, and 48 h. Results: Increased cell migration and proliferation were observed from 24 to 48 h in all the groups with significant wound closure. After 48 h, the A-PRF+ and EMD group showed complete wound closure and the highest proliferation rate compared to either EMD or A-PRF+ alone, and distilled water. Conclusion: The combined application of A-PRF+ and EMD promoted enhanced wound healing, surpassing the effects of EMD and A-PRF+ used individually. The study also demonstrated the superior ability of EMD alone in periodontal fibroblast healing. Cell migration and proliferation propensity of A-PRF+ endorse it as an option for regenerative therapy.