Comparative Expression Profile of RFRP-3 in Hypothalamus and Testis of Adult Rats

Asim Chaudhuri; Pradip Kumar Das; Probal Ranjan Ghosh; Joydip Mukherjee; Kinsuk Das; Partha Das; Dipak Banerjee

doi:10.18805/IJAR.B-4713

Comparative Expression Profile of RFRP-3 in Hypothalamus and Testis of Adult Rats

Asim Chaudhuri
, Pradip Kumar Das
, Probal Ranjan Ghosh
, Joydip Mukherjee
, Kinsuk Das
, Partha Das
, Dipak Banerjee^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Background: The present study focused on the localization and comparative expression analysis of RFRP-3 gene in the hypothalamus and testis of adult male rats. Methods: Brain and testis tissue samples were collected from adult Wister rats and used for immunohistochemistry for identification and localization RFRP-3 producing neurons. The expression level of the RFRP-3 gene was assessed by Real-time PCR and compared with muscle tissue. Result: The results confirmed the presence of RFRP-3 secreting neurons in the hypothalamus and in the testes. Scattered RFRP-3 in cell bodies was identified primarily in the VMH, DMH and surrounding the third ventricle of the brain. In testis, RFRP-3 was detected in Leydig cells, Sertoli cells and germ cells. The expression level of RFRP-3 gene in the hypothalamus was observed to be significantly higher in hypothalamus than in testicular tissue (p<0.01) of adult rats.

Original language	English
Pages (from-to)	708-713
Number of pages	6
Journal	Indian Journal of Animal Research
Volume	57
Issue number	6
DOIs	https://doi.org/10.18805/IJAR.B-4713
Publication status	Published - 06-2023

All Science Journal Classification (ASJC) codes

Animal Science and Zoology
General Veterinary

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title = "Comparative Expression Profile of RFRP-3 in Hypothalamus and Testis of Adult Rats",

abstract = "Background: The present study focused on the localization and comparative expression analysis of RFRP-3 gene in the hypothalamus and testis of adult male rats. Methods: Brain and testis tissue samples were collected from adult Wister rats and used for immunohistochemistry for identification and localization RFRP-3 producing neurons. The expression level of the RFRP-3 gene was assessed by Real-time PCR and compared with muscle tissue. Result: The results confirmed the presence of RFRP-3 secreting neurons in the hypothalamus and in the testes. Scattered RFRP-3 in cell bodies was identified primarily in the VMH, DMH and surrounding the third ventricle of the brain. In testis, RFRP-3 was detected in Leydig cells, Sertoli cells and germ cells. The expression level of RFRP-3 gene in the hypothalamus was observed to be significantly higher in hypothalamus than in testicular tissue (p<0.01) of adult rats.",

author = "Asim Chaudhuri and Das, \{Pradip Kumar\} and Ghosh, \{Probal Ranjan\} and Joydip Mukherjee and Kinsuk Das and Partha Das and Dipak Banerjee",

year = "2023",

month = jun,

doi = "10.18805/IJAR.B-4713",

language = "English",

volume = "57",

pages = "708--713",

journal = "Indian Journal of Animal Research",

issn = "0367-6722",

publisher = "Agricultural Research Communication Centre",

number = "6",

}

TY - JOUR

T1 - Comparative Expression Profile of RFRP-3 in Hypothalamus and Testis of Adult Rats

AU - Chaudhuri, Asim

AU - Das, Pradip Kumar

AU - Ghosh, Probal Ranjan

AU - Mukherjee, Joydip

AU - Das, Kinsuk

AU - Das, Partha

AU - Banerjee, Dipak

PY - 2023/6

Y1 - 2023/6

N2 - Background: The present study focused on the localization and comparative expression analysis of RFRP-3 gene in the hypothalamus and testis of adult male rats. Methods: Brain and testis tissue samples were collected from adult Wister rats and used for immunohistochemistry for identification and localization RFRP-3 producing neurons. The expression level of the RFRP-3 gene was assessed by Real-time PCR and compared with muscle tissue. Result: The results confirmed the presence of RFRP-3 secreting neurons in the hypothalamus and in the testes. Scattered RFRP-3 in cell bodies was identified primarily in the VMH, DMH and surrounding the third ventricle of the brain. In testis, RFRP-3 was detected in Leydig cells, Sertoli cells and germ cells. The expression level of RFRP-3 gene in the hypothalamus was observed to be significantly higher in hypothalamus than in testicular tissue (p<0.01) of adult rats.

AB - Background: The present study focused on the localization and comparative expression analysis of RFRP-3 gene in the hypothalamus and testis of adult male rats. Methods: Brain and testis tissue samples were collected from adult Wister rats and used for immunohistochemistry for identification and localization RFRP-3 producing neurons. The expression level of the RFRP-3 gene was assessed by Real-time PCR and compared with muscle tissue. Result: The results confirmed the presence of RFRP-3 secreting neurons in the hypothalamus and in the testes. Scattered RFRP-3 in cell bodies was identified primarily in the VMH, DMH and surrounding the third ventricle of the brain. In testis, RFRP-3 was detected in Leydig cells, Sertoli cells and germ cells. The expression level of RFRP-3 gene in the hypothalamus was observed to be significantly higher in hypothalamus than in testicular tissue (p<0.01) of adult rats.

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UR - https://www.scopus.com/pages/publications/85163801244#tab=citedBy

U2 - 10.18805/IJAR.B-4713

DO - 10.18805/IJAR.B-4713

M3 - Article

AN - SCOPUS:85163801244

SN - 0367-6722

VL - 57

SP - 708

EP - 713

JO - Indian Journal of Animal Research

JF - Indian Journal of Animal Research

IS - 6

ER -

Comparative Expression Profile of RFRP-3 in Hypothalamus and Testis of Adult Rats

Abstract

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