TY - JOUR
T1 - Crystal violet decolorization assay
T2 - a simplified colorimetric test for the rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates
AU - Reghunath, Aswathy
AU - Shenoy, Vishnu Prasad
AU - Kushal, Shouri
AU - Pandey, Akhilesh Kumar
N1 - Funding Information:
We thank Dr. Vandana K E, Professor, and Head, of the Department of Microbiology, Kasturba Medical College, Manipal for her valuable support and motivation. We are thankful to Mr. Avinash U P, Ms. Jyothsna Bai S, Ms. Ashalatha, and Mr. Sathish Nayak, the non-teaching staff, Department of Microbiology, Kasturba Medical College, Manipal who helped us by providing all the requirements to complete our work.
Funding Information:
Kasturba Medical College Manipal, Manipal Academy of Higher Education Manipal, has funded this work - Grant No. PGR582.
Publisher Copyright:
© 2023 Institut Pasteur
PY - 2023/7/1
Y1 - 2023/7/1
N2 - The increased prevalence of multi-drug resistant M. tuberculosis is quite possibly the direst and most difficult task for the early diagnosis and treatment. A rapid, reliable, and inexpensive diagnostic method is the need of the hour. The current study on crystal violet decolorization assay explores the possibility to develop a rapid and simple detection method to detect multi-drug-resistant tuberculosis isolates by comparing the results with the traditional liquid culture drug susceptibility testing method based on their sensitivity, specificity, positive predictive value, and negative predictive value. 70 isolates were used for the study and were detected as multi-drug resistant, mono drug-resistant, and sensitive by using crystal violet decolourization assay and further compared with the results of DST and using H37Rv as the standard control strain. The sensitivity, specificity, positive predictive value, and negative predictive value of crystal violet decolorization assay (Rifampicin: 100%, 94.60%, 100% and 82.40%; isoniazid: 100%, 94.10%, 100%, 86.40%) are calculated and the percentage were compared with the conventional liquid culture drug susceptibility testing for M. tuberculosis using rifampicin and isoniazid. Crystal violet decolourization assay is rapid, reproducible, and doesn't require any highly experienced personal or sophisticated laboratory instruments for interpretation. This assay is found to be nearly as reliable as conventional liquid culture drug susceptibility testing and may thus be of great help in phenotypic confirmation of multi-drug resistant tuberculosis by providing results more rapidly.
AB - The increased prevalence of multi-drug resistant M. tuberculosis is quite possibly the direst and most difficult task for the early diagnosis and treatment. A rapid, reliable, and inexpensive diagnostic method is the need of the hour. The current study on crystal violet decolorization assay explores the possibility to develop a rapid and simple detection method to detect multi-drug-resistant tuberculosis isolates by comparing the results with the traditional liquid culture drug susceptibility testing method based on their sensitivity, specificity, positive predictive value, and negative predictive value. 70 isolates were used for the study and were detected as multi-drug resistant, mono drug-resistant, and sensitive by using crystal violet decolourization assay and further compared with the results of DST and using H37Rv as the standard control strain. The sensitivity, specificity, positive predictive value, and negative predictive value of crystal violet decolorization assay (Rifampicin: 100%, 94.60%, 100% and 82.40%; isoniazid: 100%, 94.10%, 100%, 86.40%) are calculated and the percentage were compared with the conventional liquid culture drug susceptibility testing for M. tuberculosis using rifampicin and isoniazid. Crystal violet decolourization assay is rapid, reproducible, and doesn't require any highly experienced personal or sophisticated laboratory instruments for interpretation. This assay is found to be nearly as reliable as conventional liquid culture drug susceptibility testing and may thus be of great help in phenotypic confirmation of multi-drug resistant tuberculosis by providing results more rapidly.
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U2 - 10.1016/j.micinf.2023.105108
DO - 10.1016/j.micinf.2023.105108
M3 - Article
C2 - 36736854
AN - SCOPUS:85163886405
SN - 1286-4579
VL - 25
JO - Microbes and Infection
JF - Microbes and Infection
IS - 6
M1 - 105108
ER -
