TY - JOUR
T1 - Curcumin nanocrystals attenuate cyclophosphamide-induced testicular toxicity in mice
AU - Poojary, Keerthana Karunakar
AU - Nayak, Guruprasad
AU - Vasani, Ashna
AU - Kumari, Sandhya
AU - Dcunha, Reyon
AU - Kunhiraman, Jyolsna Ponnaratta
AU - Gopalan, Divya
AU - Rao, Rajat Radhakrishna
AU - Mutalik, Srinivas
AU - Kalthur, Sneha Guruprasad
AU - Murari, M. S.
AU - Raghu, Shamprasad Varija
AU - Adiga, Satish Kumar
AU - Kalthur, Guruprasad
N1 - Funding Information:
Authors thankfully acknowledge the partial financial support from the Indian Council of Medical Research (ICMR, 5/10/FR/5/2015-RCH ). SVR is grateful to DBT-Ramalingaswami Fellowship .
Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2021/12/15
Y1 - 2021/12/15
N2 - The cancer therapy using cyclophosphamide (CP) has been associated with adverse effects on the testicular function that raises concerns about the future fertility potential among cancer survivors. Curcumin, a polyphenol, has shown to possess a plethora of biological functions including tissue protective effects. In the present study, we investigated the protective effects of curcumin nanocrystals (NC) in mitigation of CP-induced testicular toxicity. Healthy adult (8–10 week) and prepubertal (2 week) male Swiss albino mice were injected with a single dose of CP (200 mg/kg) intraperitoneally (i.p). NC (4 mg/kg, i.p.) was administered every alternate day, for 35 days in adult mice while, a single dose of NC was injected intraperitoneally to prepubertal mice 1 h prior to CP. Administration of multiple doses of NC ameliorated CP-induced testicular toxicity in adult mice, which was evident from the improved sperm functional competence, sperm chromatin condensation, seminiferous tubule architecture and decreased apoptosis in testicular cells. Further, administration of NC 1 h prior to CP in prepubertal mice modulated the expression of genes pertaining to proliferation, pluripotency, DNA damage and DNA repair in spermatogonial cells at 24 h after the treatment. Overall, these results suggest that NC could be a promising chemoprotective agent, which can have potential application in male fertility preservation.
AB - The cancer therapy using cyclophosphamide (CP) has been associated with adverse effects on the testicular function that raises concerns about the future fertility potential among cancer survivors. Curcumin, a polyphenol, has shown to possess a plethora of biological functions including tissue protective effects. In the present study, we investigated the protective effects of curcumin nanocrystals (NC) in mitigation of CP-induced testicular toxicity. Healthy adult (8–10 week) and prepubertal (2 week) male Swiss albino mice were injected with a single dose of CP (200 mg/kg) intraperitoneally (i.p). NC (4 mg/kg, i.p.) was administered every alternate day, for 35 days in adult mice while, a single dose of NC was injected intraperitoneally to prepubertal mice 1 h prior to CP. Administration of multiple doses of NC ameliorated CP-induced testicular toxicity in adult mice, which was evident from the improved sperm functional competence, sperm chromatin condensation, seminiferous tubule architecture and decreased apoptosis in testicular cells. Further, administration of NC 1 h prior to CP in prepubertal mice modulated the expression of genes pertaining to proliferation, pluripotency, DNA damage and DNA repair in spermatogonial cells at 24 h after the treatment. Overall, these results suggest that NC could be a promising chemoprotective agent, which can have potential application in male fertility preservation.
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U2 - 10.1016/j.taap.2021.115772
DO - 10.1016/j.taap.2021.115772
M3 - Article
AN - SCOPUS:85118337284
SN - 0041-008X
VL - 433
JO - Toxicology and Applied Pharmacology
JF - Toxicology and Applied Pharmacology
M1 - 115772
ER -