TY - JOUR
T1 - Cyclooxygenase-2-765G>C functional promoter polymorphism and its association with oral squamous cell carcinoma.
AU - Lakshmi, Addala
AU - Muralidhar, Sadhnani
AU - Kalyan Kumar, Chennaboina
AU - Pavan Kumar, Addala
AU - Kalyana Chakravarthy, Pentapati
AU - Anjaneyulu, Vasala
AU - Kaiser, Jamil
PY - 2012/8/1
Y1 - 2012/8/1
N2 - Cyclooxygenase-2 is a key enzyme in the conversion of arachidonic acid to prostaglandins, and has critical role in the progression of several malignancies, including oral squamous cell carcinoma. We designed a case-control study to evaluate the susceptibility of the functional -765G>C genetic variation in oral squamous cell carcinoma patients. Polymerase chain reaction-based restriction fragment length polymorphism analysis was used to determine the polymorphism in oral squamous cell carcinoma (n = 150) patients and healthy controls (n = 150). The genotype frequencies of cyclooxygenase-2 765G>G, 765G>C, and 765C>C were 73.3%, 18.66%, and 8.0% in the cancer patients, and 94.66%, 4% and 1.3% in the controls, respectively. The cyclooxygenase-2 GC and CC genotypes were significantly associated (P = 0.0003 and P = 0.01, respectively) with oral squamous cell carcinoma patients, when compared to the controls. The 765G>C genotypes were statistically significant, with habitual tobacco chewing and alcohol consumption + smoking (P = 0.05). This study highlights the genetic variant that might play a role in mediating susceptibility to oral squamous cell carcinoma in this population.
AB - Cyclooxygenase-2 is a key enzyme in the conversion of arachidonic acid to prostaglandins, and has critical role in the progression of several malignancies, including oral squamous cell carcinoma. We designed a case-control study to evaluate the susceptibility of the functional -765G>C genetic variation in oral squamous cell carcinoma patients. Polymerase chain reaction-based restriction fragment length polymorphism analysis was used to determine the polymorphism in oral squamous cell carcinoma (n = 150) patients and healthy controls (n = 150). The genotype frequencies of cyclooxygenase-2 765G>G, 765G>C, and 765C>C were 73.3%, 18.66%, and 8.0% in the cancer patients, and 94.66%, 4% and 1.3% in the controls, respectively. The cyclooxygenase-2 GC and CC genotypes were significantly associated (P = 0.0003 and P = 0.01, respectively) with oral squamous cell carcinoma patients, when compared to the controls. The 765G>C genotypes were statistically significant, with habitual tobacco chewing and alcohol consumption + smoking (P = 0.05). This study highlights the genetic variant that might play a role in mediating susceptibility to oral squamous cell carcinoma in this population.
UR - http://www.scopus.com/inward/record.url?scp=84876434498&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876434498&partnerID=8YFLogxK
M3 - Article
C2 - 22887904
AN - SCOPUS:84876434498
SN - 2041-1618
VL - 3
SP - 182
EP - 188
JO - Journal of investigative and clinical dentistry
JF - Journal of investigative and clinical dentistry
IS - 3
ER -