Cytotoxic effects of Chloroform-methanol extract, protein extract and various fractions of ethanolic extract of Curcuma amada rhizome on HCT116 colon cancer cell line

Vaibhav S. Patil; B. S. Varashree; K. Sreedhara Ranganath Pai; Gangadhar Hari; Keerthi Priya

doi:10.52711/0974-360X.2022.00833

Cytotoxic effects of Chloroform-methanol extract, protein extract and various fractions of ethanolic extract of Curcuma amada rhizome on HCT116 colon cancer cell line

Vaibhav S. Patil, B. S. Varashree^*, K. Sreedhara Ranganath Pai, Gangadhar Hari, Keerthi Priya

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Cytotoxic effects of chloroform-methanol extract, protein extract and various fractions of ethanolic extracts of Curcuma amada rhizome were investigated on Human colorectal cancer cell line (HCT116). The ethanolic extract showed excellent cytotoxicity of all extracts with an IC50 value of 14.46µg followed by the Chloroform-methanol extract with an IC50 value of 18.31µg. The various fractions of ethanolic extracts fractionated with petroleum ether, ethyl acetate, n-butanol were also tested for cytotoxicity independently. Ethyl acetate fraction gave an IC50 value of 1012µg and n-butanol IC50 value was 43.19µg, with petroleum ether fraction giving no cytotoxicity. The crude proteins isolated from the rhizome showed good cytotoxicity and gave an IC50 of 43.1µg.

Original language	English
Pages (from-to)	4955-4958
Number of pages	4
Journal	Research Journal of Pharmacy and Technology
Volume	15
Issue number	11
DOIs	https://doi.org/10.52711/0974-360X.2022.00833
Publication status	Published - 11-2022

All Science Journal Classification (ASJC) codes

Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Pharmacology (medical)

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10.52711/0974-360X.2022.00833

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title = "Cytotoxic effects of Chloroform-methanol extract, protein extract and various fractions of ethanolic extract of Curcuma amada rhizome on HCT116 colon cancer cell line",

abstract = "Cytotoxic effects of chloroform-methanol extract, protein extract and various fractions of ethanolic extracts of Curcuma amada rhizome were investigated on Human colorectal cancer cell line (HCT116). The ethanolic extract showed excellent cytotoxicity of all extracts with an IC50 value of 14.46µg followed by the Chloroform-methanol extract with an IC50 value of 18.31µg. The various fractions of ethanolic extracts fractionated with petroleum ether, ethyl acetate, n-butanol were also tested for cytotoxicity independently. Ethyl acetate fraction gave an IC50 value of 1012µg and n-butanol IC50 value was 43.19µg, with petroleum ether fraction giving no cytotoxicity. The crude proteins isolated from the rhizome showed good cytotoxicity and gave an IC50 of 43.1µg.",

author = "Patil, {Vaibhav S.} and Varashree, {B. S.} and {Sreedhara Ranganath Pai}, K. and Gangadhar Hari and Keerthi Priya",

year = "2022",

month = nov,

doi = "10.52711/0974-360X.2022.00833",

language = "English",

volume = "15",

pages = "4955--4958",

journal = "Research Journal of Pharmacy and Technology",

issn = "0974-3618",

publisher = "A and V Publication",

number = "11",

}

Patil, VS, Varashree, BS , Sreedhara Ranganath Pai, K, Hari, G & Priya, K 2022, 'Cytotoxic effects of Chloroform-methanol extract, protein extract and various fractions of ethanolic extract of Curcuma amada rhizome on HCT116 colon cancer cell line', Research Journal of Pharmacy and Technology, vol. 15, no. 11, pp. 4955-4958. https://doi.org/10.52711/0974-360X.2022.00833

Cytotoxic effects of Chloroform-methanol extract, protein extract and various fractions of ethanolic extract of Curcuma amada rhizome on HCT116 colon cancer cell line. / Patil, Vaibhav S.; Varashree, B. S.; Sreedhara Ranganath Pai, K. et al.
In: Research Journal of Pharmacy and Technology, Vol. 15, No. 11, 11.2022, p. 4955-4958.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cytotoxic effects of Chloroform-methanol extract, protein extract and various fractions of ethanolic extract of Curcuma amada rhizome on HCT116 colon cancer cell line

AU - Patil, Vaibhav S.

AU - Varashree, B. S.

AU - Sreedhara Ranganath Pai, K.

AU - Hari, Gangadhar

AU - Priya, Keerthi

PY - 2022/11

Y1 - 2022/11

N2 - Cytotoxic effects of chloroform-methanol extract, protein extract and various fractions of ethanolic extracts of Curcuma amada rhizome were investigated on Human colorectal cancer cell line (HCT116). The ethanolic extract showed excellent cytotoxicity of all extracts with an IC50 value of 14.46µg followed by the Chloroform-methanol extract with an IC50 value of 18.31µg. The various fractions of ethanolic extracts fractionated with petroleum ether, ethyl acetate, n-butanol were also tested for cytotoxicity independently. Ethyl acetate fraction gave an IC50 value of 1012µg and n-butanol IC50 value was 43.19µg, with petroleum ether fraction giving no cytotoxicity. The crude proteins isolated from the rhizome showed good cytotoxicity and gave an IC50 of 43.1µg.

AB - Cytotoxic effects of chloroform-methanol extract, protein extract and various fractions of ethanolic extracts of Curcuma amada rhizome were investigated on Human colorectal cancer cell line (HCT116). The ethanolic extract showed excellent cytotoxicity of all extracts with an IC50 value of 14.46µg followed by the Chloroform-methanol extract with an IC50 value of 18.31µg. The various fractions of ethanolic extracts fractionated with petroleum ether, ethyl acetate, n-butanol were also tested for cytotoxicity independently. Ethyl acetate fraction gave an IC50 value of 1012µg and n-butanol IC50 value was 43.19µg, with petroleum ether fraction giving no cytotoxicity. The crude proteins isolated from the rhizome showed good cytotoxicity and gave an IC50 of 43.1µg.

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Cytotoxic effects of Chloroform-methanol extract, protein extract and various fractions of ethanolic extract of Curcuma amada rhizome on HCT116 colon cancer cell line

Abstract

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