TY - JOUR
T1 - Cytotoxic effects of Chloroform-methanol extract, protein extract and various fractions of ethanolic extract of Curcuma amada rhizome on HCT116 colon cancer cell line
AU - Patil, Vaibhav S.
AU - Varashree, B. S.
AU - Sreedhara Ranganath Pai, K.
AU - Hari, Gangadhar
AU - Priya, Keerthi
N1 - Publisher Copyright:
© RJPT All right reserved.
PY - 2022/11
Y1 - 2022/11
N2 - Cytotoxic effects of chloroform-methanol extract, protein extract and various fractions of ethanolic extracts of Curcuma amada rhizome were investigated on Human colorectal cancer cell line (HCT116). The ethanolic extract showed excellent cytotoxicity of all extracts with an IC50 value of 14.46µg followed by the Chloroform-methanol extract with an IC50 value of 18.31µg. The various fractions of ethanolic extracts fractionated with petroleum ether, ethyl acetate, n-butanol were also tested for cytotoxicity independently. Ethyl acetate fraction gave an IC50 value of 1012µg and n-butanol IC50 value was 43.19µg, with petroleum ether fraction giving no cytotoxicity. The crude proteins isolated from the rhizome showed good cytotoxicity and gave an IC50 of 43.1µg.
AB - Cytotoxic effects of chloroform-methanol extract, protein extract and various fractions of ethanolic extracts of Curcuma amada rhizome were investigated on Human colorectal cancer cell line (HCT116). The ethanolic extract showed excellent cytotoxicity of all extracts with an IC50 value of 14.46µg followed by the Chloroform-methanol extract with an IC50 value of 18.31µg. The various fractions of ethanolic extracts fractionated with petroleum ether, ethyl acetate, n-butanol were also tested for cytotoxicity independently. Ethyl acetate fraction gave an IC50 value of 1012µg and n-butanol IC50 value was 43.19µg, with petroleum ether fraction giving no cytotoxicity. The crude proteins isolated from the rhizome showed good cytotoxicity and gave an IC50 of 43.1µg.
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U2 - 10.52711/0974-360X.2022.00833
DO - 10.52711/0974-360X.2022.00833
M3 - Article
AN - SCOPUS:85144727261
SN - 0974-3618
VL - 15
SP - 4955
EP - 4958
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 11
ER -