TY - JOUR
T1 - Cytotoxicity and radiosensitizing potency of Moscatilin in cancer cells at low radiation doses of X-ray and UV-C
AU - Pujari, Ipsita
AU - Thomas, Abitha
AU - Thomas, Jinsu
AU - Jhawar, Niharika
AU - Guruprasad, Kanive Parashiva
AU - Rai, Padmalatha S.
AU - Satyamoorthy, Kapaettu
AU - Babu, Vidhu Sankar
N1 - Funding Information:
The authors thank the Science and Engineering Research Board-Extra Mural Research (SERB-EMR) (presently called Core Research Grant [CRG]), Government of India, File No. EMR/2015/001816 for funding this research work. Both IP and AT have equally contributed to this article as first authors. IP and AT thank Manipal Academy of Higher Education (MAHE), Manipal, India, for providing the prestigious Dr T. M. A. Pai PhD Scholarship. IP acknowledges SERB for granting the Junior Research Fellowship (JRF) from May, 2017 to December 2019. The authors also thank Dr. BS Satish Rao, Director-Research, MAHE, and Manipal to provide the facility for irradiation experiments. The authors thank Mrs. Rekha Nayak, Department of Cell and Molecular Biology, MSLS, MAHE, Manipal, India for the technical assistance provided during cell line studies and Mrs. Jyothi Nayak, Department of Ageing Research, MSLS, MAHE, Manipal, India for the technical assistance in flow cytometry.
Funding Information:
The authors thank the Science and Engineering Research Board-Extra Mural Research (SERB-EMR) (presently called Core Research Grant [CRG]), Government of India, File No. EMR/2015/001816 for funding this research work. Both IP and AT have equally contributed to this article as first authors. IP and AT thank Manipal Academy of Higher Education (MAHE), Manipal, India, for providing the prestigious Dr T. M. A. Pai PhD Scholarship. IP acknowledges SERB for granting the Junior Research Fellowship (JRF) from May, 2017 to December 2019. The authors also thank Dr. BS Satish Rao, Director-Research, MAHE, and Manipal to provide the facility for irradiation experiments. The authors thank Mrs. Rekha Nayak, Department of Cell and Molecular Biology, MSLS, MAHE, Manipal, India for the technical assistance provided during cell line studies and Mrs. Jyothi Nayak, Department of Ageing Research, MSLS, MAHE, Manipal, India for the technical assistance in flow cytometry.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/6
Y1 - 2021/6
N2 - Moscatilin (stilbenoid) is a plant-derived anticancer compound, and it has mostly been isolated from threatened wild Dendrobium species. The present study attempts to evaluate the cytotoxicity of Moscatilin on several cancer cell lines through MTT assay. Additionally, it also aims towards estimating and comparing the radiosensitivity, cell-cycle progression, and apoptotic/necrotic effect induced by Moscatilin on different cell lines. The effects of Moscatilin was compared with another significant stilbenoid anticancer agent, Resveratrol (a structural analog of Moscatilin), whose presence has also been reported in Dendrobiums. Considering the threatened nature of this genus, crude extracts of a tropical and epiphytic Dendrobium species, viz., Dendrobium ovatum, prepared from in vitro seedlings were also tested towards cytotoxicity and radiosensitization efficacy. Moscatilin functioned as an effective radiosensitizer at 5 µg/ml along with 1 Gy X-ray and 200 J/m2 UV-C radiations. It was also able to perturb cell cycle both at replicative and post-replicative phases with the aforementioned combination. Moscatilin, in unison with radiation, triggered immunogenic death specifically on cancer cells starting from Pyroptosis, terminating in Necroptosis. Moscatilin, when used singly, could evoke immunogenic cell death. Analyses of Damage-Associated Molecular Patterns released during radiation and Moscatilin treatment would aid in ascertaining the mode of cell death. Moscatilin is a potential radiosensitizer and must be tested for preclinical and clinical trials to combat cancer.
AB - Moscatilin (stilbenoid) is a plant-derived anticancer compound, and it has mostly been isolated from threatened wild Dendrobium species. The present study attempts to evaluate the cytotoxicity of Moscatilin on several cancer cell lines through MTT assay. Additionally, it also aims towards estimating and comparing the radiosensitivity, cell-cycle progression, and apoptotic/necrotic effect induced by Moscatilin on different cell lines. The effects of Moscatilin was compared with another significant stilbenoid anticancer agent, Resveratrol (a structural analog of Moscatilin), whose presence has also been reported in Dendrobiums. Considering the threatened nature of this genus, crude extracts of a tropical and epiphytic Dendrobium species, viz., Dendrobium ovatum, prepared from in vitro seedlings were also tested towards cytotoxicity and radiosensitization efficacy. Moscatilin functioned as an effective radiosensitizer at 5 µg/ml along with 1 Gy X-ray and 200 J/m2 UV-C radiations. It was also able to perturb cell cycle both at replicative and post-replicative phases with the aforementioned combination. Moscatilin, in unison with radiation, triggered immunogenic death specifically on cancer cells starting from Pyroptosis, terminating in Necroptosis. Moscatilin, when used singly, could evoke immunogenic cell death. Analyses of Damage-Associated Molecular Patterns released during radiation and Moscatilin treatment would aid in ascertaining the mode of cell death. Moscatilin is a potential radiosensitizer and must be tested for preclinical and clinical trials to combat cancer.
UR - http://www.scopus.com/inward/record.url?scp=85106861382&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85106861382&partnerID=8YFLogxK
U2 - 10.1007/s13205-021-02827-3
DO - 10.1007/s13205-021-02827-3
M3 - Article
AN - SCOPUS:85106861382
SN - 2190-572X
VL - 11
JO - 3 Biotech
JF - 3 Biotech
IS - 6
M1 - 281
ER -
