TY - JOUR
T1 - Dengue NS1 antigen detection using photoluminescence of solution phase biotylinated anti-NS1 antibody conjugated ZnO quantum dots
AU - Namboothiri, Pramod K.
AU - Govindan, Sreejith
AU - Rajitha, K. V.
AU - Jasmine, Sajna
AU - Peter, Mathew
AU - Niranjan, D. B.
AU - Umakanth, Shashikiran
N1 - Funding Information:
This work was supported by Manipal Centre for Infectious Diseases (MAC-ID), MAHE, Manipal (MAC ID/SGA/2017/17, 2018).
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/3/1
Y1 - 2022/3/1
N2 - Biotinylated anti-NS1 antibody of dengue was conjugated on the streptavidin functionalized ZnO quantum dots (QD) by EDC-NHS chemistry. Photoluminescence spectra of the ZnO QDs showed band edge peak (∼366 nm, peak 1) and a defect related peak (∼470 nm, peak 2). The defect related peak was present only for Zn/Li ratio below 0.5. The change in the luminescence intensity of peak 1 and peak 2 was studied with negative and positive serum of suspected dengue cases. A 14% decrease of the average intensity of peak 1 and peak 2 was observed per 20 μl addition of negative serum. In the case of dengue positive serum, the average intensity was increased by 7% per 20 μl of serum sample. This suggests that the prepared solution phase ZnO QDs could be applied in the detection of dengue antigen in suspected patients’ serum.
AB - Biotinylated anti-NS1 antibody of dengue was conjugated on the streptavidin functionalized ZnO quantum dots (QD) by EDC-NHS chemistry. Photoluminescence spectra of the ZnO QDs showed band edge peak (∼366 nm, peak 1) and a defect related peak (∼470 nm, peak 2). The defect related peak was present only for Zn/Li ratio below 0.5. The change in the luminescence intensity of peak 1 and peak 2 was studied with negative and positive serum of suspected dengue cases. A 14% decrease of the average intensity of peak 1 and peak 2 was observed per 20 μl addition of negative serum. In the case of dengue positive serum, the average intensity was increased by 7% per 20 μl of serum sample. This suggests that the prepared solution phase ZnO QDs could be applied in the detection of dengue antigen in suspected patients’ serum.
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U2 - 10.1016/j.matchemphys.2022.125778
DO - 10.1016/j.matchemphys.2022.125778
M3 - Article
AN - SCOPUS:85123735951
SN - 0254-0584
VL - 279
JO - Materials Chemistry and Physics
JF - Materials Chemistry and Physics
M1 - 125778
ER -
