Biotinylated anti-NS1 antibody of dengue was conjugated on the streptavidin functionalized ZnO quantum dots (QD) by EDC-NHS chemistry. Photoluminescence spectra of the ZnO QDs showed band edge peak (∼366 nm, peak 1) and a defect related peak (∼470 nm, peak 2). The defect related peak was present only for Zn/Li ratio below 0.5. The change in the luminescence intensity of peak 1 and peak 2 was studied with negative and positive serum of suspected dengue cases. A 14% decrease of the average intensity of peak 1 and peak 2 was observed per 20 μl addition of negative serum. In the case of dengue positive serum, the average intensity was increased by 7% per 20 μl of serum sample. This suggests that the prepared solution phase ZnO QDs could be applied in the detection of dengue antigen in suspected patients’ serum.
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Condensed Matter Physics