TY - JOUR
T1 - Design of ECM Functionalized Polycaprolactone Aligned Nanofibers for Peripheral Nerve Tissue Engineering
AU - Nune, Manasa
AU - Bhat, Mahima
AU - Nagarajan, Aishwarya
N1 - Funding Information:
Open access funding provided by Manipal Academy of Higher Education, Manipal. The authors would like to acknowledge the Department of Science & Technology for the SERB start-up research Grant (SRG/2019/002130) for financial support. We would also like to thank the MAHE seed money grant for faculty research.
Funding Information:
The authors would like to acknowledge the Department of Science & Technology for the SERB start-up research grant (SRG/2019/002130) and Manipal Academy of Higher Education (MAHE) seed money grant for faculty research for financial support. We would also like to thank Ms. Srividya H and Ms. Michelle Abraham for the help during material preparation and cell culture experiments. We also would extend thanks to the Manipal Institute of Regenerative Medicine for the infrastructural support.
Funding Information:
The authors would like to acknowledge the Department of Science & Technology for the SERB start-up research grant (SRG/2019/002130) and Manipal Academy of Higher Education (MAHE) seed money grant for faculty research for financial support. We would also like to thank Ms. Srividya H and Ms. Michelle Abraham for the help during material preparation and cell culture experiments. We also would extend thanks to the Manipal Institute of Regenerative Medicine for the infrastructural support.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/4
Y1 - 2022/4
N2 - Purpose: Peripheral nerve injury (PNI) and its regeneration continue to remain a significant medical burden worldwide. The current treatment strategies used to treat PNI are often associated with multiple complications and yet do not achieve complete motor and sensory functions. Recently, synthetic biodegradable nerve conduits have become one the most commonly used conduits to repair small gaps in nerve injury. But they have not shown better results than nerve grafts possibly because of the lack of biological microenvironment required for axonal growth. Schwann cells play a very crucial role in peripheral nerve regeneration where activated SCs produce multiple neurotrophic factors that help in remyelination and immune modulation during nerve repair. Studies have shown that nanofibrous scaffolds have better bioactivity and more closely mimic the native structure of the extracellular matrix. Therefore, the present study was focused on designing a nanofibrous scaffold that would cover the roles of both structural support for the cells that can provide a microenvironment with biological cues for nerve growth and regeneration. Methods: Decellularized Schwann cell ECM were spin-coated on polycaprolactone random and aligned nanofibrous scaffolds and their compatibility was evaluated using Schwann cells. Results: Schwann cells displayed growth in the direction of the aligned PCL nanofibers and ACM treated exhibited appropriate bipolar morphology indicating that these modified fibers could provide directional cues making them highly suitable for neuronal cell growth. Conclusion: Our results indicate that the fabricated aligned SC-ACM treated PCL scaffolds would be a potential biomaterial to treat peripheral nerve injuries and promote regeneration. Graphical Abstract: [Figure not available: see fulltext.]
AB - Purpose: Peripheral nerve injury (PNI) and its regeneration continue to remain a significant medical burden worldwide. The current treatment strategies used to treat PNI are often associated with multiple complications and yet do not achieve complete motor and sensory functions. Recently, synthetic biodegradable nerve conduits have become one the most commonly used conduits to repair small gaps in nerve injury. But they have not shown better results than nerve grafts possibly because of the lack of biological microenvironment required for axonal growth. Schwann cells play a very crucial role in peripheral nerve regeneration where activated SCs produce multiple neurotrophic factors that help in remyelination and immune modulation during nerve repair. Studies have shown that nanofibrous scaffolds have better bioactivity and more closely mimic the native structure of the extracellular matrix. Therefore, the present study was focused on designing a nanofibrous scaffold that would cover the roles of both structural support for the cells that can provide a microenvironment with biological cues for nerve growth and regeneration. Methods: Decellularized Schwann cell ECM were spin-coated on polycaprolactone random and aligned nanofibrous scaffolds and their compatibility was evaluated using Schwann cells. Results: Schwann cells displayed growth in the direction of the aligned PCL nanofibers and ACM treated exhibited appropriate bipolar morphology indicating that these modified fibers could provide directional cues making them highly suitable for neuronal cell growth. Conclusion: Our results indicate that the fabricated aligned SC-ACM treated PCL scaffolds would be a potential biomaterial to treat peripheral nerve injuries and promote regeneration. Graphical Abstract: [Figure not available: see fulltext.]
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U2 - 10.1007/s40846-022-00699-3
DO - 10.1007/s40846-022-00699-3
M3 - Article
AN - SCOPUS:85127939215
SN - 1609-0985
VL - 42
SP - 147
EP - 156
JO - Journal of Medical and Biological Engineering
JF - Journal of Medical and Biological Engineering
IS - 2
ER -