TY - JOUR
T1 - Development and validation of a HPLC method for quantification of rivastigmine in rat urine and identification of a novel metabolite in urine by LC-MS/MS
AU - Arumugam, Karthik
AU - Chamallamudi, Mallikarjuna Rao
AU - Gilibili, Ravindranath Reddy
AU - Mullangi, Ramesh
AU - Ganesan, Subramanian
AU - Kar, Sidhartha S.
AU - Averineni, Ranjithkumar
AU - Shavi, Gopal
AU - Udupa, Nayanabhirama
PY - 2011/3
Y1 - 2011/3
N2 - A sensitive, specific and accurate HPLC method for the quantification of rivastigmine (RSM) in rat urine was developed and validated. The method involves the simple liquid-liquid extraction of RSM and pyridostigmine as an internal standard (IS) from rat urine with tertiary methyl butyl ether. The chromatographic separation of RSM and IS was achieved with 20mm ammonium acetate buffer (pH 6.5) and acetonitrile (65:35, v/v) delivered at flow-rate of 1mL/min on a Kromasil KR-100. The method was in linear range from 50 to 5000ng/mL. The validation was done as per FDA guidelines and the results met the acceptance criteria. The method was successfully applied for the quantification of RSM in rat urine. Besides method validation, we have identified two metabolites of RSM in urine. Both the metabolites were characterized by HPLC-PDA and LC-MS/MS and it was found that one metabolite is novel.
AB - A sensitive, specific and accurate HPLC method for the quantification of rivastigmine (RSM) in rat urine was developed and validated. The method involves the simple liquid-liquid extraction of RSM and pyridostigmine as an internal standard (IS) from rat urine with tertiary methyl butyl ether. The chromatographic separation of RSM and IS was achieved with 20mm ammonium acetate buffer (pH 6.5) and acetonitrile (65:35, v/v) delivered at flow-rate of 1mL/min on a Kromasil KR-100. The method was in linear range from 50 to 5000ng/mL. The validation was done as per FDA guidelines and the results met the acceptance criteria. The method was successfully applied for the quantification of RSM in rat urine. Besides method validation, we have identified two metabolites of RSM in urine. Both the metabolites were characterized by HPLC-PDA and LC-MS/MS and it was found that one metabolite is novel.
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U2 - 10.1002/bmc.1455
DO - 10.1002/bmc.1455
M3 - Article
C2 - 20540167
AN - SCOPUS:79951542374
SN - 0269-3879
VL - 25
SP - 353
EP - 361
JO - Biomedical Chromatography
JF - Biomedical Chromatography
IS - 3
ER -