Development of HPLC assay method for the estimation of olanzapine in human plasma and its application to pharmacokinetics

B. Dasandi; P. Shah; C. Gandhi; K. M. Bhat; Shivprakash

Development of HPLC assay method for the estimation of olanzapine in human plasma and its application to pharmacokinetics

B. Dasandi
, P. Shah
, C. Gandhi
, K. M. Bhat^*
, Shivprakash

^*Corresponding author for this work

Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A new method for the estimation of olanzapine in human plasma is developed and validated. It utilizes RP select B column and amperometric detector. The mobile phase consisted of 75mM sodium dihydrogen phosphate buffer of pH 7.0, acetonitrile and methanol in 55:22.5:22.5 %v/v ratios in an isocratic mode at 1.5ml^-1 flow rate. The drug is extracted in to 3ml each of Tertiary Butyl Methyl Ether (TBME) twice from plasma, evaporated and the residue dissolved in mobile phase. The percentage extraction is found to be more than 79% and the linearity range from 0.5ng to 50.0ng ml^-1. The method has been validated extensively and found to have good interday and intraday precision. This method has been applied to study pharmacokinetics in 12 healthy human volunteers.

Original language	English
Pages (from-to)	350-354
Number of pages	5
Journal	Indian Drugs
Volume	40
Issue number	6
Publication status	Published - 01-06-2003

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmaceutical Science
Drug Discovery

Cite this

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title = "Development of HPLC assay method for the estimation of olanzapine in human plasma and its application to pharmacokinetics",

abstract = "A new method for the estimation of olanzapine in human plasma is developed and validated. It utilizes RP select B column and amperometric detector. The mobile phase consisted of 75mM sodium dihydrogen phosphate buffer of pH 7.0, acetonitrile and methanol in 55:22.5:22.5 \%v/v ratios in an isocratic mode at 1.5ml-1 flow rate. The drug is extracted in to 3ml each of Tertiary Butyl Methyl Ether (TBME) twice from plasma, evaporated and the residue dissolved in mobile phase. The percentage extraction is found to be more than 79\% and the linearity range from 0.5ng to 50.0ng ml-1. The method has been validated extensively and found to have good interday and intraday precision. This method has been applied to study pharmacokinetics in 12 healthy human volunteers.",

author = "B. Dasandi and P. Shah and C. Gandhi and Bhat, \{K. M.\} and Shivprakash",

year = "2003",

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day = "1",

language = "English",

volume = "40",

pages = "350--354",

journal = "Indian Drugs",

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publisher = "Indian Drug Manufacturers' Association",

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TY - JOUR

T1 - Development of HPLC assay method for the estimation of olanzapine in human plasma and its application to pharmacokinetics

AU - Dasandi, B.

AU - Shah, P.

AU - Gandhi, C.

AU - Bhat, K. M.

AU - Shivprakash,

PY - 2003/6/1

Y1 - 2003/6/1

N2 - A new method for the estimation of olanzapine in human plasma is developed and validated. It utilizes RP select B column and amperometric detector. The mobile phase consisted of 75mM sodium dihydrogen phosphate buffer of pH 7.0, acetonitrile and methanol in 55:22.5:22.5 %v/v ratios in an isocratic mode at 1.5ml-1 flow rate. The drug is extracted in to 3ml each of Tertiary Butyl Methyl Ether (TBME) twice from plasma, evaporated and the residue dissolved in mobile phase. The percentage extraction is found to be more than 79% and the linearity range from 0.5ng to 50.0ng ml-1. The method has been validated extensively and found to have good interday and intraday precision. This method has been applied to study pharmacokinetics in 12 healthy human volunteers.

AB - A new method for the estimation of olanzapine in human plasma is developed and validated. It utilizes RP select B column and amperometric detector. The mobile phase consisted of 75mM sodium dihydrogen phosphate buffer of pH 7.0, acetonitrile and methanol in 55:22.5:22.5 %v/v ratios in an isocratic mode at 1.5ml-1 flow rate. The drug is extracted in to 3ml each of Tertiary Butyl Methyl Ether (TBME) twice from plasma, evaporated and the residue dissolved in mobile phase. The percentage extraction is found to be more than 79% and the linearity range from 0.5ng to 50.0ng ml-1. The method has been validated extensively and found to have good interday and intraday precision. This method has been applied to study pharmacokinetics in 12 healthy human volunteers.

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UR - https://www.scopus.com/pages/publications/11144254518#tab=citedBy

M3 - Article

AN - SCOPUS:11144254518

SN - 0019-462X

VL - 40

SP - 350

EP - 354

JO - Indian Drugs

JF - Indian Drugs

IS - 6

ER -

Development of HPLC assay method for the estimation of olanzapine in human plasma and its application to pharmacokinetics

Abstract

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All Science Journal Classification (ASJC) codes

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